Supercharge Your Innovation With Domain-Expert AI Agents!

Aspergillus clavatus Ac-32 strain producing lovastatin and its application

A technology of ac-32 and lovastatin, applied in the field of biotechnology microorganisms, can solve the problems of lack of excellent industrial strains of lovastatin, etc., and achieve the effects of large spore production, fast growth rate and simple cultivation.

Active Publication Date: 2018-08-03
ZHEJIANG NORMAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In response to the increasing demand for Lovastatin in the market, the lack of excellent industrial strains for producing Lovastatin, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aspergillus clavatus Ac-32 strain producing lovastatin and its application
  • Aspergillus clavatus Ac-32 strain producing lovastatin and its application
  • Aspergillus clavatus Ac-32 strain producing lovastatin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation, screening and identification of Aspergillus clavatus (Aspergillus clavatus) Ac-32 bacterial strain

[0028] 1 medium

[0029] ①PDA medium: 20% potato, 2% glucose, 2.0% agar powder, 1L water, pH 5.5-6.0. For the isolation, purification and identification of Aspergillus strains.

[0030] ②PD medium: 20% potato, 2% glucose, 0.5% beef extract, 1L water, pH 5.5-6.0. For the screening of Lovastatin-producing Aspergillus strains.

[0031] 2 Experimental methods

[0032] 2.1 Isolation and purification of Aspergillus strains

[0033] Pick a small amount of mycelia from the surface of natural fermentation samples (food, soil, organic matter, etc.) collected from different habitats and insert them on the surface of the PDA medium plate, and cultivate them at 28°C for 24 hours. After being connected to another PDA medium plate to continue culturing for 3 days to produce spores, the typical characteristics of Aspergillus were observed under a microscope, an...

Embodiment 2

[0046] The growth curve and Lovastatin production rule of embodiment 2 Aspergillus clavatus (Aspergillus clavatus) Ac-32 strain in 5L fermentor

[0047] 1 strain: Ac-32 Aspergillus strain.

[0048] 2 media

[0049] ① PDA medium: the formula is the same as in Example 1.

[0050] ②PD medium: the formula is the same as that in Example 1.

[0051] ③Seed medium: lactose 10%, glycerol 1%, peptone 0.5%, soybean flour 1%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.1%, NaNO 3 0.2%, water 1L, pH 5.5.

[0052] ④Fermentation medium: lactose 20%, glycerin 2%, soybean peptone 1%, yeast extract 0.5%, MgSO 4 ·7H 2 O0.1%, NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 0.2%, water 1L, pH 5.2.

[0053] 3 Experimental methods

[0054] 3.1 Cultivation of Ac-32 strain

[0055] Use an inoculation needle to pick a small amount of Ac-32 strain mycelium, transfer it to a PDA medium plate, and activate it for 3 days; take 1 bacteria cake (0.8 mm in diameter) from the plate and inoculate it in PD medium,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
lengthaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biotechnology microorganisms, and in particular relates to an Aspergillus clavatus Ac-32 strain for producing Lovastatin by liquid fermentation, a use and a fermentation production method. The strain of Aspergillus clavatus (Aspergillus clavatus) Ac-32 screened by the present invention has excellent characteristics such as high yield of Lovastatin, fast growth, large amount of spore production, simple cultivation and the like. Under the condition that the initial pH of the fermentation medium was 5.2 and the culture temperature was 28°C, after the Ac-32 strain was optimized by liquid fermentation medium and fermentation conditions, the production of Lovastatin could reach 200 mg / L~250 mg / L or so.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, and in particular relates to an Aspergillus clavatus (Aspergillus clavatus) Ac-32 strain for producing Lovastatin by liquid fermentation, a use and a fermentation production method. Background technique [0002] In recent years, the prevalence, incidence and risk factors of cardiovascular disease in the Chinese population have continued to rise. According to the latest data from the National Disease Surveillance System, the proportion of cardiovascular disease in total deaths in my country has increased from 37.5% to 40.7%. Lovastatin, a secondary metabolite of Aspergillus, has become one of the best-selling drugs for the treatment of cardiovascular diseases due to its clear mechanism of action and remarkable clinical efficacy in the prevention and treatment of hyperlipidemia, atherosclerosis and cardiovascular and cerebrovascular diseases . [0003] In 1979, Japanese scholar Akira En...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/66
Inventor 蒋冬花章婷任浩戴梦怡郑逸舒
Owner ZHEJIANG NORMAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com