Method of detecting galectin-1 in blood and reagent therefor

A technology for galectin and blood, which is applied in the field of detecting galectin-1 in blood, can solve the problems of low absolute content, little help in early detection and intervention treatment of cancer metastasis, lack of detection methods, etc., and achieves high sensitivity Effect

Active Publication Date: 2016-01-13
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the blood galectin-1 concentration of cancer patients is higher than that of normal people, its absolute content is still very low (ng / ml level), and there is still a lack of effective and convenient detection methods for early detection and intervention of cancer metastasis little help

Method used

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  • Method of detecting galectin-1 in blood and reagent therefor
  • Method of detecting galectin-1 in blood and reagent therefor
  • Method of detecting galectin-1 in blood and reagent therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1, Preparation of lactose-modified gold nanorod detection probes

[0053] In this example, the seed-induced gold nanorod synthesis method is used to prepare gold nanorods with good dispersion performance, single particle size, suitable long-short axis ratio, and stable existence; synthesize suitable lactose ligands; Lactose ligands were loaded onto the surface of gold nanorods to achieve lactosylation on the surface of gold nanorods, and a lactose-modified gold nanorod detection probe was prepared and its structure was confirmed.

[0054] 1. Preparation of gold nanorods

[0055] Gold rods were prepared by seed-induced synthesis of gold nanorods in aqueous solution. First reduce the chloroauric acid with sodium borohydride to prepare 1-2nm gold seeds, then add the gold nano-seeds to the chloroauric acid (HAuCl 4 ), silver nitrate (AgNO 3 ), dilute hydrochloric acid (HCl), ascorbic acid, and dodecyldimethylammonium bromide (CTAB) in a mixed aqueous solution to ...

Embodiment 2

[0080] Example 2, the detection of galectin-1 by molecular level gold nanorods (Lac-GNRs)

[0081] This example evaluates the detection of galectin-1 at the molecular level.

[0082] The method used to detect galectin-1: In operation, add 100 microliters of the substance to be tested (aqueous solution of galectin-1) to 1 milliliter of the Lac-GNR aqueous solution prepared in Example 1, shake well and let stand at room temperature for one hours, use a UV-Vis-NISspectrophotometer (UV-Vis-NISspectrophotometer) to optically detect the system, measure the light absorption from 250nm to 1100nm, and judge whether the substance to be tested contains galectin-1 through the change of the absorption curve, or even It can be judged with the naked eye by the change in color.

[0083] The detection result of this embodiment finds that Lac-GNRs can detect the existence of galectin-1 extremely sensitively, and the detection limit can reach 1pM, and the response value changes with the change ...

Embodiment 3

[0085] Example 3. Detection of galectin-1 by Lac-GNRs at the cellular level

[0086] In this example, the detection of galectin-1 is studied at the cellular level, because galectin-1 is a secreted protein, which will be secreted into the extratumor matrix to play a role. The cancer cells are cultured by the method of cell culture, and the culture fluid thereof is taken for detection, and the existence of galectin-1 should be detected.

[0087] In this example, HUH-7 (human liver cancer cells), MCF-7 (human breast cancer cells), JEG-3 (human choriocarcinoma cells), HeLa (human cervical cancer cells), Hep-G2 (human liver cancer cells) were cultured in total. Cells), C6 (mouse glioma cells) and other six kinds of tumor cells, and their cell culture fluids were tested. The culture conditions of the cells are: the cells are planted in the H-DMEM culture medium containing 10% fetal bovine serum (high-glucose Dulbecco's minimum essential medium, the cell concentration is about 2×10 ...

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Abstract

The invention provides a method of detecting galectin-1 in to-be-tested samples, especially in blood, and a reagent therefor. The method includes the steps of: contacting and mixing gold nano rods, which is modified by lactose, as a probe with the to-be-tested sample; maintaining the system temperature at 20-37 DEG C; allowing the system to stand for 10-120 min; and detecting the galectin-1 by observing the color of the mixture system. In the invention, with the gold nano rods modified by lactose (Lac-GNRs) as the probe and ultraviolet-visible light-near infrared spectrophotometer as detection method, high-effective and specific detection to the galectin-1 is carried out at the molecular and cellular levels. The method can accurately detect the galectin-1 in human blood and is an effective method of monitoring early stage of cancer metastasis.

Description

technical field [0001] The invention relates to a method for detecting galectin-1 in blood and a reagent used therefor. Background technique [0002] Cancer is a disease that seriously threatens human life and health around the world. According to statistics, more than 7 million people die of cancer in the world every year (Ahmedin, J., Rebecca S., Elizabeth W., Hao, Y.P., Xu, J.Q., Taylor, M ., Michael, J.T. Cancer Statistics, CA: Cancer J. Clin. 2008, 58, 71-96). The incidence of cancer in my country is on a linear upward trend, and a large number of patients are already in the middle and late stages when they seek treatment, basically losing the possibility of cure. An important reason why patients with advanced cancer are difficult to cure is that cancer is prone to metastasis. The primary tumor can be removed surgically or treated with radiation, but cancer that has spread is often difficult to treat without damaging normal tissue. Tumor metastasis is accompanied by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 赵岳涛喻学锋潘浩波王怀雨
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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