Device for analyzing cells and monitoring cell culturing and method for analyzing cells and monitoring cell culturing using same

Abstract

The present invention relates to a device for analyzing cells or monitoring a cell culturing process and a method for analyzing cells and monitoring cell culturing using the same. A device for analyzing cells and monitoring cell culturing according to the present invention has a camera module of MN matrix type at the lower end of a support, which contains a cell incubator, thereby making it possible to apply a multi-well cell incubator, such as a 96 well plate, and simultaneously analyze the change of various cells with respect to various culturing conditions or external environment changes, for example. Specifically, the device can selectively switch on camera modules in a specific area, thereby enabling partial observation; the device can macroscopically observe the entire well or microscopically observe an area of interest of one well at a high magnification; the device can observe the behavior image of cells in real time; the device can solve the problem of pollution while performing cell analysis and culturing; the device can remotely check the culturing state in real time.

Description

technical field [0001] The present invention generally relates to a system for analyzing cells and monitoring cell culture and a method for analyzing cells and monitoring cell culture using the system, and more particularly relates to a system for analyzing cells and monitoring cell culture. The MxN matrix camera module below the culture unit in which the cell culture vessel is placed, so that changes in cells cultured in multi-hole cell culture vessels such as 96-well plates (by various changes in culture conditions or external environments) can be analyzed simultaneously. caused by such changes), and relates to a method for analyzing cells and monitoring cell culture using the system for analyzing cells and monitoring cell culture. Specifically, the system according to the present invention can realize the observation of the desired monitoring part by selectively opening only the camera module corresponding to the desired monitoring part, and can microscopically observe all ...

AI Technical Summary

Problems solved by technology

[0005] However, this conventional method of observing cells during culture has a drawback in that the viability of the cells is destroyed, since observing cells should be done as quickly as possible and the cells should be returned to the incubator after the observation is complete

In addition, there is a problem that it is difficult to achieve accurate evaluation due to the unstable activity of the cells

Moreover, when cells are removed from the incubator, contamination of the cells with toxic substances is unavoidable

Also, since cultured cells are usually colorless and transparent, these cells are difficult to observe with a transmission electron microscope

[0006] In addition, in order to observe or describe cells in culture according to the conventional method, both the incubator and the cell culture dish or bottle need to be opened, and for this reason, the culture conditions will be destroyed, making it impossible to observe or describe cells in real time during the culture process

Moreover, the observation or depiction of cells depends on the skills of the observers, and observation errors between different observers are unavoidable

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