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Method for measuring factors affecting rhizosphere soil amino acid availability

A rhizosphere soil and amino acid technology, which is applied in the field of determining the factors affecting the availability of rhizosphere soil amino acids, can solve the problems of heavy experimental workload, difficulty in accurately evaluating soil interaction, and difficult realization, and achieves the effect of simple operation

Active Publication Date: 2016-01-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These factors are complex, and it is difficult to accurately evaluate the interaction in soil to study the influence of a single factor on the contribution of amino acid nutrition, and the workload of multi-factor and multi-level experiments is large and difficult to achieve.

Method used

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  • Method for measuring factors affecting rhizosphere soil amino acid availability
  • Method for measuring factors affecting rhizosphere soil amino acid availability
  • Method for measuring factors affecting rhizosphere soil amino acid availability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0021] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

Embodiment 2

[0027] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0028] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

Embodiment 3

[0034] (1) Soil sample collection: Collect 15 representative zonal soils. The collection points are located in the typical soil type zone. The brick red soil is located in Haikou, Hainan, the red soil is located in Zhanjiang, Guangdong, and the red soil is located in Shangrao, Jiangxi (two samples), Guangxi Nanning, yellow-brown soil in Sheqi, Henan, yellow soil in Anshun, Guizhou, purple soil in Suining, Sichuan (two samples), fluvo-aquic soil in Minquan, Henan, brown soil in Changzhi, Shanxi, brown soil in Yantai and Shenyang Tieling, dark brown soil It is located in Huadian, Jilin, and the black soil is located in Harbin. When sampling, first use a shovel to remove the litter layer of the topsoil, take 5kg of the topsoil of 0-15cm, and accurately record the location of the sampling point, planting system and vegetation type;

[0035] (2) Sample pre-treatment: After picking out the stones and root system, the sample passes through a 4mm sieve and placed in a ventilated and dr...

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Abstract

The invention provides a method for measuring factors affecting rhizosphere soil amino acid availability. The method comprises the steps that typical zonal soil is collected for sample pretreatment comprising screening and grinding, the soil is placed into an artificial greenhouse for culturing plants, labeled amino acid is added, the soil plant sample treatment is performed to measure the physicochemical property of the soil, linear correlation analysis is carried out on the physicochemical property of the soil and the amino acid absorptive amount, and the main soil factors affecting the soil amino acid biological validity are researched. The method is easy to implement, the factors affecting the amino acid biological availability significantly can be found from complex factors through correlation detection, and a method foundation is provided for researching and controlling amino acid biological availability.

Description

technical field [0001] The invention belongs to the field of soil ecology, in particular to a method for measuring factors affecting the availability of amino acids in rhizosphere soil. Background technique [0002] Soil contains a variety of nitrogen-containing compounds, such as inorganic nitrogen, amino acids, peptides and proteins. In addition to inorganic nitrogen, amino acids can also be used as an important nitrogen source for plant growth. Chapin et al. (1993) found for the first time that the vascular plants without mycorrhizae like to absorb amino acids. The amount of amino acids absorbed by the mycorrhizal plants of the arctic tundra, the white wool beard grass, accounted for at least 60% of the total absorbed nitrogen, and amino acids were used as the only nitrogen source. The nitrogen absorbed and the biomass formed during cultivation were more than those when inorganic nitrogen was used as nitrogen source. Many researchers have conducted short-term root absorp...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N33/50G01N33/24
Inventor 马庆旭吴良欢
Owner ZHEJIANG UNIV
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