Petrocosmea sinensis tissue culture and rapid propagation method

A Chinese stone butterfly, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of difficult to achieve large-scale cultivation, resource depletion, limited scope, etc., to shorten the cultivation time and improve production. speed, the effect of promoting rooting

Inactive Publication Date: 2016-02-10
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The geographical distribution of the Chinese stone butterfly is limited, and it grows on the rocks in the shade of low mountains. In recent years, with the increasing market demand and folk excavation, its growth environment has been damaged to varying degrees, and its resources are on the verge of exhaustion. It is difficult to realize large-scale cultivation in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] An example of the Chinese stone butterfly tissue culture rapid propagation method of the present invention, comprises the steps:

[0019] (1) Selection and disinfection of explants: Take the young leaves of Chinese stone butterfly as explants, rinse the explants slowly under running water, and gently wipe the dirt on the surface with a soft brush, and put them into a beaker In the process, soak in 2v / v% detergent for 15 minutes, and then rinse with running water for 10 minutes. Move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 to 100ml of 0.1v / v% HgCl 2 Disinfect for 8, 12, and 15 minutes, soak in sterile water for 3 times, inoculate after drying the surface moisture of the explants with sterile filter paper, wherein the sterile water is distilled water sterilized by high temperature and high pressure.

[0020] (2) Induction of adventitious buds: Inoculate the explants obtained in step ...

Embodiment 2

[0026] An example of the Chinese stone butterfly tissue culture rapid propagation method of the present invention, comprises the steps:

[0027] (1) Selection and disinfection of explants: Take the young leaves of Chinese stone butterfly as explants, rinse the explants slowly under running water, and gently wipe the dirt on the surface with a soft brush, and put them into a beaker In the process, soak in 2v / v% detergent for 15 minutes, and then rinse with running water for 10 minutes. Move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 to 100ml of 0.1v / v% HgCl 2 Disinfect for 8, 12, and 15 minutes, soak in sterile water for 3 times, inoculate after drying the surface moisture of the explants with sterile filter paper, wherein the sterile water is distilled water sterilized by high temperature and high pressure.

[0028] (2) Induction of adventitious buds: Inoculate the explants obtained in step ...

Embodiment 3

[0034] An example of the Chinese stone butterfly tissue culture rapid propagation method of the present invention, comprises the steps:

[0035] (1) Selection and disinfection of explants: Take the young leaves of Chinese stone butterfly as explants, rinse the explants slowly under running water, and gently wipe the dirt on the surface with a soft brush, and put them into a beaker In the process, soak in 2v / v% detergent for 15 minutes, and then rinse with running water for 10 minutes. Move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then add 1-2 drops of Tween-20 to 100ml of 0.1v / v% HgCl 2 Disinfect for 8, 12, and 15 minutes, soak in sterile water for 3 times, inoculate after drying the surface moisture of the explants with sterile filter paper, wherein the sterile water is distilled water sterilized by high temperature and high pressure.

[0036] (2) Induction of adventitious buds: Inoculate the explants obtained in step ...

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PUM

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Abstract

A petrocosmea sinensis tissue culture and rapid propagation method comprises the following steps: (1), taking tender leaves of petrocosmea sinensis as an explant and sterilizing the explant; (2), placing the sterilized explant in an MS minimal medium to induce sterile buds; (3), placing the sterile buds into an MS inducing medium for strengthening seedlings so as to obtain robust plants; (4), placing the robust plants into an MS rooting medium for culture so as to obtain complete rooted seedlings; (5), taking the complete rooted seedlings, hardening the seedlings, enabling the seedlings to grow in sandy soil for one month and then transplanting the seedlings to a field. The plants obtained by adopting the method provided by the invention are thick and strong and high in survival rate, and the method can provide a large number of petrocosmea sinensis seedlings which are high in quality and suitable for cultivation in the short term, so that the problem of scale seedling cultivation of petrocosmea sinensis is solved effectively.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant tissue culture, in particular to a method for rapid propagation of Chinese stone butterfly tissue culture. Background technique [0002] Chinese Stone Butterfly (Petrocosmeasinensis Oliv.) is a perennial herb of Gesneriaceae. It is produced in northern Yunnan, Sichuan and western Hubei, and is born on stones in the shade of low mountains. At present, there are about 140 genera and more than 2,000 species of Gesneriaceae plants in the world. There are 58 genera and 463 species in China, which are distributed from Liaoning to Hainan. Most of the genera and species are distributed in Yunnan, Guangxi and Taiwan. On the cliffs of tropical and subtropical limestone in Guangdong and other provinces. [0003] The Chinese stone butterfly is beautiful in color and has high ornamental value. At the same time, it is also a kind of traditional Chinese medicine. The whole plant is used as medicine. I...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王一诺韦坤华李翠韦莹肖冬李林轩王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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