Improved verticillium wilt resistance identification method for cotton variety in artificial disease garden during whole growth period

Abstract

The invention relates to the field of cotton verticillium wilt identification and specifically relates to an improved verticillium wilt resistance identification method for a cotton variety in an artificial disease garden during a whole growth period. The method comprises the steps that (1) pathogenic bacteria used for identification are cultivated, a corn and sand culture medium is applied, and sand and corn powder are mixed thoroughly according to a mass ratio of 1:1; (2) the disease garden is built and inoculation is carried out, wherein the inoculation of the pathogenic bacteria is carried out by a seed trench inoculation method; (3) states of cotton verticillium wilt are investigated; and (4) verticillium wilt resistance of the cotton variety is evaluated. According to the method provided by the invention, disease attacking is uniform and consistent in the disease garden, so that more accurate disease identification results can be obtained; classification standards of disease state investigation are scientific and can be mastered easily; the disease resistance of the cotton during the whole growth period can be reflected fully; and results are accurate. In addition, the method is economic and environment-friendly.

Description

technical field [0001] The invention relates to the field of identification of cotton Verticillium wilt, in particular to an improved identification method for Verticillium wilt resistance of cotton varieties in artificial disease gardens during the whole growth period. Background technique [0002] Verticillium dahliae Kleb. is a major disease in cotton production. After the 1960s, with the expansion of cotton area and frequent introduction and adjustment, cotton verticillium wilt began to spread and became more and more serious. Effective control of Verticillium wilt damage is the key to high and stable cotton yields, planting disease-resistant varieties is the most economical and effective measure to control its damage, and the core of breeding disease-resistant varieties is to accurately evaluate the disease resistance of varieties. [0003] Verticillium wilt resistance identification methods of cotton varieties are divided into seedling identification methods and adult...

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Problems solved by technology

[0005] In the process of disease resistance identification, there are some prominent problems in the adult plant stage identification method of artificial disease nursery: ① The necessary inoculation technology for establishing artificial disease nursery is immature, mainly because the inoculation amount is inaccurate (450kg-750kg per hectare), The method of inoculation is unscientific. Spreading the strains on a large area and then plowing is prone to uneven distribution of the strains, which eventually leads to uneven disease occurrence; The relationship is not close, and it cannot reflect the tolerance and disease resistance of the variety to the disease; ③The evaluation method of disease resistance is unscientific, and the disease resistance of the variety is evaluated based on the disease index at a point in the peak period of the disease, which cannot reflect the entire growth period of the cotton variety disease resistance

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