A kind of Pluzobacterium floating type bacterial liquid inoculation method

Abstract

The invention belongs to the technical field of indoor inoculation of clubroot fungi and discloses a method for inoculation of clubroot fungus floating bacterial liquid. The frozen cabbage swollen roots are taken out, thawed at room temperature, cut into small pieces, weighed, and added 5 times the weight Sterile water, use a tissue mashing homogenizer to make a homogenate, filter through 6 layers of gauze, adjust the pH to 5-6, use a hemocytometer to measure the concentration of dormant spores in the homogenate; sow the sterilized seeds on In the floating seedling tray equipped with sterilized substrate, add the inoculum into the floating tray; make the concentration of dormant spores in the mixed substrate reach 10 8 unit / g substrate, keep it moist for 48 hours under dark conditions at 25°C, and carry out normal management. The clubroot floating bacterial liquid inoculation method of the present invention is designed for a quick and uniform inoculation method, so that the onset of the disease is fast after inoculation, the growth of the variety is uniform, the onset is uniform, the sensitivity of disease discrimination is high, and the true disease resistance and disease resistance of the variety can be correctly classified. The purpose is simple and convenient operation.

Description

technical field [0001] The invention belongs to the technical field of indoor inoculation of Phytophthora rhizogenes, and particularly relates to a floating bacterial liquid inoculation method of Phytophthora rhizogenes. Background technique [0002] At present, the prior art commonly used in the industry is as follows: establishing a stable artificial disease inoculation method is the basis for screening cruciferous materials against clubroot. The existing indoor inoculation methods of P. rhizogenes mainly include rhizosphere soil injection method, soil mixing method, root irrigation method, and root dipping method. The root irrigation method is to inoculate the dormant spore suspension of P. rhizogenes around the host root system when the seedlings grow to two leaves and one center. The method is to pull out the root system and wash it with clean water during the two-leaf and one-heart stage, and then inoculate it with the bacterial suspension. Although the bacterial sour...

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Problems solved by technology

[0004] (1) The root irrigation inoculation method is to pour the dormant spore suspension of Plasmodium root around the host root system when the seedlings grow to two leaves and one heart, but the disadvantage is that the amount of swollen roots used is large, which wastes the inoculation source and wastes manpower for operation.

[0005] (2) The root dipping inoculation method is to pull out the root system and wash it with clean water at the stage of two leaves and one heart, and then dip the bacterial suspension to inoculate. Although the source of bacteria can be saved, the operation is more complicated

[0006] (3) The rhizosphere soil injection method is characterized by individual injection and inoculation in the seedling holes one by one after sowing, the workload is cumbersome, time-consuming and laborious

[0007] (4) The soil mixing method is to mix the prepared Plasmodium dormant spore suspension into the aseptic seedling substrate, which will easily cause uneven concentration of the inoculum solution in the substrate and affect the statistical results.

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