Composition for preventing hair loss or promoting hair growth containing barley bark extract as an active ingredient
A technology of hair growth and composition, which is applied in the field of anti-hair loss or hair growth-promoting composition, which can solve the problems of preventing hair loss without barley skin, and achieve the effect of inhibiting and preventing hair loss and promoting hair growth
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Embodiment 1
[0108] The acquisition and extraction of barley skin
[0109] The barley husk used in the present invention is the barley husk of Chinese wheat cultivated in Gyeongsangbuk-do, Korea, which is obtained from rice milling. Use 90% ethanol every 4 hours to decoct 5 kg of the total wheat husk sample in a water bath (Water Bath) and extract it three times. Thereafter, the supernatant was recovered and concentrated under reduced pressure using a rotary evaporator to obtain 337 g of a crude extract. Of these, 300 g of the mixture was subjected to solvent fractionation with n-hexane (n-hexane) to obtain 133 g of an n-hexane layer, and the fractionated product was used as a sample for the experiment.
Embodiment 2
[0110] Proliferation efficacy of dermal papilla cells
Embodiment 2-1
[0111] Confirmation of proliferation of dermal papilla cells
[0112] Immortalized dermal papilla cells (Rat vibrissa immortalized dermal papilla cells) isolated from the whiskers of white mice were treated with 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal Bovine serum (heat-inactivated fetal bovine serum, FBS; Gibco Inc, NY, USA) in DMEM (Hyclone Inc, USA) medium, at 37°C, 5% CO 2 Cultured in a constant temperature machine, subcultured every 3 days.
[0113] The proliferation of dermal papilla cells was measured by MTT Assay. In DMEM medium containing 1% FBS, dermal papilla cells (dermal papilla cell) 1.0×10 4 The cells / ml were turbid and put into a 96-well plate (well plate). After culturing for 24 hours, the wheat peel hexane was fractionated at concentrations of 0.1, 1, 10, 50 and 100 μg / ml. Minoxidil (minoxidil, Sigma, MO, USA) as a positive control group was treated with a concentration of 10 μM.
[0114] After c...
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