Dialysis bag isolated culture method for researching correlation among microorganisms

Abstract

The invention relates to a method for researching co-culture of different types of microorganisms. The method is characterized by respectively sealing a small amount of culture mediums containing different types of microorganisms into dialysis bags with specific apertures in an aseptic environment, soaking the sealed dialysis bags into a liquid culture medium, and culturing. The method can be utilized for simultaneously culturing multiple microorganisms without influencing exchange between various bacteria and nutrient substances and bacterial metabolism substances in the culture medium; after the culture is finished and the bacteria-containing culture mediums in the dialysis bags are taken out in the aseptic environment are subjected to viable count, the growth number of each bacteria can be accurately calculated, and antagonism or commensalism among the different microorganisms can be further researched; besides, by selecting the dialysis bags with different apertures, bacterial proteins entering or exiting dialysis bags can be controlled, and the physiological action of bacterial proteins of different sizes to other bacteria can be researched. The method can be used for simultaneously researching correlation among multiple microorganisms and is simple and rapid.

Description

technical field [0001] The invention relates to a method for studying the co-cultivation of different types of microorganisms, which can more simply and conveniently study the interaction between different microorganisms, and is of great significance for studying the interaction of various microorganisms in different habitats, especially in probiotic products. Background technique [0002] In recent years, research on probiotics in the human gut has become more and more popular, and the development of probiotic products has become more rapid. Probiotics are a kind of healthy bacteria that are safe, non-toxic and side effects, and can resist the invasion of foreign pathogens. Therefore, the research on the relationship between probiotics and other pathogenic bacteria is also more important, and it is also of great significance to the development of probiotic products. . [0003] Common methods for studying the interaction between bacteria include bacterial co-culture method ...

AI Technical Summary

Problems solved by technology

However, when studying the relationship between bacteria, the bacterial mixed co-cultivation method can fully contact different kinds of bacteria but cannot accurately determine the number of different bacteria in the culture solution. The result is difficult to count and it is inconvenient to analyze the experimental results.

The Oxford cup method is to obtain a substance produced by the activity of a bacterium, and study its inhibitory effect on another bacterium after diffusing through the Oxford cup. However, the bacteria have no direct contact and only rely on the related substances produced by the bacterial activity, which leads to the experimental Result decreased sensitivity