Dialysis bag isolated culture method for researching correlation among microorganisms

A technology of microorganisms and dialysis bags, applied in the field of co-cultivation of different types of microorganisms, can solve the problems of decreased sensitivity of experimental results, inconvenient analysis of experimental results, inability to accurately determine the number of different bacteria, etc., achieving convenient cultivation methods, easy statistics and analysis. Effect

Inactive Publication Date: 2016-04-13
NANCHANG UNIV
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AI Technical Summary

Problems solved by technology

However, when studying the relationship between bacteria, the bacterial mixed co-cultivation method can fully contact different kinds of bacteria but cannot accurately determine the number of different bacteria in the culture solution. The result is difficult to count and it is inconvenient to analyze the experimental results.
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The cultivation method of the present invention comprises the following steps:

[0021] (1) pH value detection

[0022] a Measure about 50ml of deionized sterile water, add concentrated hydrochloric acid, and use a pH regulator to prepare a solution with pH = 4

[0023] b Take 200ml of deionized sterile water and put it into beaker A1, add 5ml of the above pH=4 solution at the same time, use a pH meter to detect the pH in beaker A1; 1000kd dialysis bag and seal both ends with dialysis bag clips, place the dialysis bag in beakers B1, C1, D1, E1 of 200ml deionized sterile water respectively, measure the pH value in the above four beakers with a pH meter, record B1 , C1, D1, E1 beaker pH value reaches the time of A1 beaker pH value, recorded as time T 1 .

[0024] (2) Glucose detection

[0025] a Prepare 50ml of 25% glucose solution with deionized sterile water

[0026] b Take 200ml of deionized sterile water and add it to beaker A2, add 5ml of the above-mentioned glu...

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Abstract

The invention relates to a method for researching co-culture of different types of microorganisms. The method is characterized by respectively sealing a small amount of culture mediums containing different types of microorganisms into dialysis bags with specific apertures in an aseptic environment, soaking the sealed dialysis bags into a liquid culture medium, and culturing. The method can be utilized for simultaneously culturing multiple microorganisms without influencing exchange between various bacteria and nutrient substances and bacterial metabolism substances in the culture medium; after the culture is finished and the bacteria-containing culture mediums in the dialysis bags are taken out in the aseptic environment are subjected to viable count, the growth number of each bacteria can be accurately calculated, and antagonism or commensalism among the different microorganisms can be further researched; besides, by selecting the dialysis bags with different apertures, bacterial proteins entering or exiting dialysis bags can be controlled, and the physiological action of bacterial proteins of different sizes to other bacteria can be researched. The method can be used for simultaneously researching correlation among multiple microorganisms and is simple and rapid.

Description

technical field [0001] The invention relates to a method for studying the co-cultivation of different types of microorganisms, which can more simply and conveniently study the interaction between different microorganisms, and is of great significance for studying the interaction of various microorganisms in different habitats, especially in probiotic products. Background technique [0002] In recent years, research on probiotics in the human gut has become more and more popular, and the development of probiotic products has become more rapid. Probiotics are a kind of healthy bacteria that are safe, non-toxic and side effects, and can resist the invasion of foreign pathogens. Therefore, the research on the relationship between probiotics and other pathogenic bacteria is also more important, and it is also of great significance to the development of probiotic products. . [0003] Common methods for studying the interaction between bacteria include bacterial co-culture method ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/06
CPCC12N1/20C12Q1/06
Inventor 陈廷涛辛洪波孟凡景王鑫
Owner NANCHANG UNIV
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