On-line digestion combined with liquid chromatography system and detection method of colorants in tea

Abstract

The invention provides an on-line digestion combined liquid chromatography system and a detection method for colorants in tea leaves. The system includes a first flow path, a second flow path, a waste liquid flow path, an analysis flow path, a switching valve, etc., and also includes a digestion flow path channel, digestion solution channel, buffer channel, and extraction channel, etc. The system can realize the online extraction of the dyes that need to be digested in tea leaves or other solid matrices, without the cumbersome process of offline digestion, extraction, and concentration, and realize the integration and high automation of extraction and liquid chromatography analysis, greatly improving work efficiency. save time. The detection method of the invention is used for on-line detection of other dyes that do not need to be digested in tea, and different dyes can be detected with the same set of equipment.

Description

technical field [0001] The invention belongs to the field of liquid chromatographic systems, and in particular relates to an on-line digestion combined liquid chromatographic system and a detection method for colorants in tea. Background technique [0002] Liquid chromatography has the advantages of high detection sensitivity, high separation efficiency, high selectivity, low detection limit, less sample consumption, convenience and quickness, etc., especially combined with mass spectrometry detector, which greatly improves detection sensitivity and anti-interference ability, and has been widely used In the safety testing of the food industry, such as the detection of natural toxins, pesticides, food additives, veterinary drugs, etc. in food. [0003] Generally, no coloring agents are added to tea, but some unscrupulous traders will add some coloring agents to improve the color and appearance of tea, such as phthalocyanine blue, phthalocyanine red, phthalocyanine green G and...

AI Technical Summary

Problems solved by technology

Doing so requires a long manual process, which is cumbersome, time-consuming and labor-intensive

[0005] In addition, the above extraction process requires extraction containers, heating appliances, ultrasonic instruments, vibration instruments and other auxiliary instruments. After the sample is processed, it needs to be manually transferred to the liquid chromatograph for analysis, and the complete integration of sample processing and liquid chromatography cannot be realized. The on-line determination of liquid chromatography greatly reduces the automation of liquid chromatography, which in turn affects the efficiency and speed of batch sample analysis.

In addition, due to the instability of the chemical structure, some target components are prone to oxidation, volatilization, decomposition and other problems, so the problem of target loss may occur during the transfer process, thus affecting the accuracy of the entire liquid chromatographic determination

[0006] In short, there is no liquid chromatography system that can realize on-line digestion and analysis in the prior art, and there is no detection method for coloring agents in tea leaves. It is of great significance to develop such a system and method

Images