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Anti-Hantaan virus mouse/human chimeric antibody and its application

A technology of Hantaan virus and human antibody, applied in the direction of antiviral agent, antibody, application, etc., can solve the problem that human monoclonal antibody cannot be produced on a large scale, reduce the incidence of human anti-mouse antibody reaction, and achieve high affinity , the effect of good clinical treatment effect

Inactive Publication Date: 2019-04-09
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0004] Therefore, the preparation of human monoclonal antibodies can prevent the occurrence of these problems, but some difficulties have also been encountered in the process of preparing human monoclonal antibodies. Large-scale production of human monoclonal antibodies

Method used

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  • Anti-Hantaan virus mouse/human chimeric antibody and its application
  • Anti-Hantaan virus mouse/human chimeric antibody and its application
  • Anti-Hantaan virus mouse/human chimeric antibody and its application

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Embodiment Construction

[0029] The present invention will be described in detail below in combination with specific embodiments.

[0030] 1. Construction of mouse / human chimeric antibody eukaryotic expression vector:

[0031] 1. Extract the anti-Hantaan virus mouse monoclonal antibody hybridoma cell line mRNA, reverse transcribe it into cDNA as a template, and use specific primers to amplify V H and V L Genes, the PCR amplification products were respectively cloned into the pUC18 vector, and sequenced to obtain the heavy chain and light chain variable region gene sequences.

[0032] 2. Clone human IgG1 heavy chain constant region and kappa light chain constant region genes from healthy human B lymphocytes, and connect the variable region and constant region by overlapping extension PCR to obtain the complete antibody gene. The light and heavy chain genes were respectively excised with corresponding restriction endonucleases, and respectively connected with expression vectors to construct mammalian ...

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Abstract

The invention relates to an anti-Hantann-virus mouse / human chimeric antibody and an application thereof. The anti-Hantann-virus mouse / human chimeric antibody comprises a heavy chain and a light chain, wherein the heavy chain comprises a mouse antibody heavy chain variable region and a human antibody heavy chain constant region, and the light chain comprises a mouse antibody light chain variable region and a human antibody light chain constant region; the mouse antibody heavy chain variable region is a heavy chain variable region of an anti-Hantann-virus mouse monoclonal antibody, and the mouse antibody light chain variable region is a light chain variable region of the anti-Hantann-virus mouse monoclonal antibody; the human antibody heavy chain constant region is a human antibody gamma 1 constant region, and the human antibody light chain constant region is a human antibody kappa chain constant region. The anti-Hantann-virus mouse / human chimeric antibody has better neutralization activity, compared with the mouse monoclonal antibody, the chimeric antibody reserves high affinity of the mouse-derived antibody variable region and has multiple immune killing functions of Fc fragments of the human antibody, so that the occurrence rate of human anti-mouse antibody response is greatly reduced, a half-life period in vivo is longer, and the chimeric antibody has better clinical treatment effect.

Description

technical field [0001] The invention relates to an antibody, in particular to an anti-Hantan virus mouse / human chimeric antibody and application thereof. Background technique [0002] The development of monoclonal antibody technology has greatly influenced the research on the treatment of human diseases. Kohler and Milstein's application of cell fusion technology to prepare monoclonal antibodies has caused a revolution in biology, and its impact is equivalent to recombinant DNA cloning technology. Monoclonal antibodies produced from hybridomas have been widely used in diagnosis and basic scientific research; in the treatment of human diseases, including viral and microbial infections, their efficacy needs to be further confirmed. [0003] Although mouse monoclonal antibodies can affect the progress of human diseases through their own specificity, due to their murine nature, their application to humans can cause immune rejection, which limits their application in the treatme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18C12N15/13A61K39/395A61P31/14A61P13/12
CPCA61K2039/505C07K16/18C07K2317/24
Inventor 吴兴安王芳张晓晓应旗康刘梓谕张芳琳程林峰于澜张亮徐志凯
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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