LYRM2 (LYR motif-containing 2) gene and new use of expression product thereof

A gene expression and gene technology, which is applied in the new application field of LYRM2 gene and its expression products in the detection of osteoporosis, can solve the problems of unclearness and lack of research results, and achieve clear results, strong selectivity and specificity. strong effect

Inactive Publication Date: 2016-04-20
BEIJING MEDINTELL BIOMED CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the etiology of osteoporosis is still not very clear, and the relevant research results are also very scarce.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • LYRM2 (LYR motif-containing 2) gene and new use of expression product thereof
  • LYRM2 (LYR motif-containing 2) gene and new use of expression product thereof
  • LYRM2 (LYR motif-containing 2) gene and new use of expression product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 High-throughput sequencing and analysis

[0031] Collect peripheral blood samples from 9 cases of osteoporosis patients and 6 cases of healthy people for RNA extraction. After RNA extraction, agarose gel electrophoresis can be used to preliminarily determine whether the quality of the extracted RNA samples is qualified or not. for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0032] The sequencing platform is the HiSeq2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) software Overall assessment of the quality of sequencing data, including base quality value distribution, quality value position distribution, GC content, PCRduplication content, kmer frequency...

Embodiment 2

[0033] Example 2 The expression of LYRM2 gene in the peripheral blood of patients with osteoporosis and the peripheral blood of healthy people

[0034] 1. Materials and methods

[0035] 1. Materials

[0036] The peripheral blood of 74 patients with osteoporosis and 46 healthy people were collected, grouped and numbered.

[0037] 2. Method

[0038] 2.1 Extraction of total RNA from peripheral blood of osteoporosis and healthy people

[0039] use Reagent was used to extract RNA from samples, and the experimental operation was performed according to the product instructions.

[0040] Judgment criteria for RNA quality: the OD260 / OD280 value of the RNA sample is between 1.8 and 2.2; the electrophoretic pattern of total RNA has clear 28S and 18S bands; difference.

[0041] 2.2 Synthesis of cDNA by reverse transcription

[0042] use IIIReverseTranscriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out...

Embodiment 3

[0060] The cultivation of embodiment 3 cell lines MG-63

[0061] 1. Materials

[0062] The cell line MG-63 was purchased from Shanghai Cell Institute, Chinese Academy of Sciences.

[0063] 2. The main solution

[0064] Cell culture medium: DMEM medium + 10% standard fetal bovine serum.

[0065] 0.25% trypsin digestion solution: add 0.25g trypsin to 100m1 deionized water, filter and sterilize, and distribute for use.

[0066] 3. Cell passage

[0067] 1 Discard the original culture medium in the culture bottle full of cells, add 1m1 of 0.25% trypsin solution to cover the cell layer, sterilize the bottle mouth, and cover it;

[0068] 2 Observe the cell changes under an inverted microscope. As time goes by, the original adherent cells gradually tend to be round, the intercellular matrix shrinks, and the intercellular space increases. Discard the trypsin before floating and add 5ml The culture solution containing 10% fetal bovine serum terminates the digestion;

[0069] 3 Cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of biomedicine, in particular to LYRM2 (LYR motif-containing 2) gene and new use of an expression product thereof. In order to solve the problem that osteoporosis molecular markers are scarce at present, the inventor screens pathogenic target genes based on the analysis of high-throughput sequencing in connection with bioinformatic methods, selects candidate gene LYRM2, verifies that LYRM2 gene is well related to osteoporosis by molecular biology experiments, and also designs an efficient interference RNA for LYRM2. The osteoporosis assisted diagnosis and treatment target provided by the invention is well worthy of clinical application.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a new application of the LYRM2 gene and its expression product, and more specifically to a new application of the LYRM2 gene and its expression product in detecting osteoporosis. Background technique [0002] Osteoporosis is a metabolic bone disease characterized by low bone mass and microarchitectural disruption of bone tissue, resulting in increased bone fragility and susceptibility to fractures. Osteoporosis has serious consequences and is a common cause of death and disability in the elderly. After fracture, most elderly patients suffer from chronic pain, permanent disability, loss of independent living ability, and severely reduced quality of life. Vertebral fractures also often cause back pain, physical deformity, shortened height, hunchback and even disability. In addition, the medical costs of osteoporosis are high, and the fractures caused by it lead to a huge occupation of m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68A61K45/00A61P19/10
CPCC12Q1/6883A61K45/00C12Q2600/158G01N33/6893G01N2333/47G01N2800/108
Inventor 林慧芳杨承刚任静
Owner BEIJING MEDINTELL BIOMED CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap