A double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunomodulatory effect and its preparation and use method

A double-stranded polynucleotide and sulfated polysaccharide technology, applied in the field of medicine, can solve problems such as antibiotic residues, achieve moderate molecular weight, strong resistance to biological enzyme degradation, and improve the effect of immune regulation activity

Active Publication Date: 2019-01-18
北京加益维科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0058] In 1969, the Chinese Academy of Sciences collaborated with relevant units to creatively use a new formulation, and developed a new polymuscular cell complex formulation, that is, adding kanamycin sulfate containing polyamide positive ions on the basis of PIC to Protect the double-strand structure from rapid hydrolysis by nucleases and maintain good stability so that it can play its due role. Because the molecular weight of kanamycin sulfate is smaller than that of polylysine with a molecular weight of tens of thousands or hundreds of thousands much, so the side effects are much lower, but the use of kanamycin is likely to cause problems such as antibiotic residues

Method used

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  • A double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunomodulatory effect and its preparation and use method
  • A double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunomodulatory effect and its preparation and use method
  • A double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunomodulatory effect and its preparation and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] a, 0.1mol / L calcium chloride solution preparation:

[0109]Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.

[0110] b. Preparation of 0.01mol / L PBS solution (pH=7.6):

[0111] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).

[0112] c. Preparation of 2mg / ml PolyIC intermediate solution:

[0113] Weigh 1.0088g of PolyI and 0.9912g of PolyC and add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, place in 45°C water bath for 60 minutes, and pass through a filter membrane with a p...

Embodiment 2

[0121] a, 0.1mol / L calcium chloride solution preparation:

[0122] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.

[0123] b. Preparation of 0.01mol / L PBS solution (pH=7.6):

[0124] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).

[0125] c. Preparation of 2mg / ml PolyIC intermediate solution:

[0126] Weigh 1.0088g of PolyI and 0.9912g of PolyC and add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, place in 45°C water bath for 60 minutes, pass through a filter membrane with a pore...

Embodiment 3

[0136] a, 0.1mol / L calcium chloride solution preparation:

[0137] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.

[0138] b. Preparation of 0.01mol / L PBS solution (pH=7.6):

[0139] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).

[0140] c, 2mg / ml preparation of bursal virus RNA solution:

[0141] The bursal virus RNA was dissolved in PBS buffer, and sterilized by filtration through a filter membrane with a pore size of 0.45 μm to prepare a 2 mg / ml solution for later use.

[0142] d. Preparation of 10mg / ml sodium heparin sulfate solution:...

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Abstract

The invention provides a double-strand polynucleotide-epsilon-polylysine-sulfuric acid glycan compound with an immune regulating function and a preparing and using method thereof. The invention further provides an immunogen composition with functions of triggering and enhancing immunoreaction and changing immunoreaction types. The immunogen composition contains the double-strand polynucleotide-epsilon-polylysine-sulfuric acid glycan compound and an antigen. The compound is formed by compounding double-strand polynucleotide and anion sulfuric acid glycan with cation epsilon-polylysine and metal cation. The compound has immune-regulating pharmacological activity. The preparing technology of the compound is simple. The compound has good water solubility and a function of resisting biological enzyme degradation. The immunogen composition can trigger and enhance the immunoreaction and change the immunoreaction types.

Description

technical field [0001] The present invention relates to the field of medicine. Specifically, the present invention provides a double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunoregulatory effect and its preparation method and application method. The present invention also provides an immunogen composition capable of triggering and enhancing immune response and changing the type of immune response and its preparation and use method. Background technique [0002] 1. Double-stranded polynucleotide: Double-stranded polynucleotide or its derivatives have various pharmacological activities, and the source is natural extraction or artificial synthesis. Artificially synthesized double-stranded polynucleotides such as polyinocytic (polyI:C) can induce the production of interferon in the body, protect cells from virus infection, and have curative effects such as preventing and treating viral diseases; naturally extracted double-stranded polynucleotides such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/12
CPCA61K39/12A61K39/39A61K2039/55511A61K2039/55583C12N2760/20134
Inventor 苏文全
Owner 北京加益维科生物科技有限公司
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