A use of tio 2 ‑Phenanthroline-loaded cyp119 enzyme photocatalytic oxidation degradation method of methyl orange

A CYP119, photocatalytic oxidation technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymes, etc., to achieve the effects of easy availability of raw materials, maintaining enzyme activity, and excellent photocatalytic oxidation performance

Inactive Publication Date: 2017-11-21
NANCHANG HANGKONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention relates to a kind of utilization TiO 2 - A method for the photocatalytic oxidation degradation of methyl orange loaded with o-phenanthroline with CYP119 enzyme, the raw material is easy to obtain, the cost is low, and the operation is simple. The method has not been reported

Method used

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  • A use of tio <sub>2</sub> ‑Phenanthroline-loaded cyp119 enzyme photocatalytic oxidation degradation method of methyl orange
  • A use of tio <sub>2</sub> ‑Phenanthroline-loaded cyp119 enzyme photocatalytic oxidation degradation method of methyl orange

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Experimental program
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Embodiment 1

[0018] Mix 3mL of n-butyl titanate with 10mL of ethanol to obtain a uniform solution A; dissolve 0.06g of phenanthroline in 10 mL of ethanol to obtain a uniform solution B; under constant stirring, slowly add solution B to In solution A, a homogeneous solution was obtained. The obtained solution was added into a hydrothermal reaction kettle, and reacted at 180° C. for 24 hours. Centrifuge the obtained solid, wash it with deionized water and ethanol alternately for more than three times, dry it, and grind it. Mix 50 mg of the finely ground powder with 20 mL of 1 uM CYP119 enzyme solution, stir at 10°C for 12 h, centrifuge, and wash to obtain the product.

Embodiment 2

[0020] Mix 6 mL of n-butyl titanate with 20 mL of ethanol to obtain a uniform solution A; dissolve 0.12 g of phenanthroline in 20 mL of ethanol to obtain a uniform solution B; under constant stirring, slowly add solution B to In solution A, a homogeneous solution was obtained. The obtained solution was added into a hydrothermal reaction kettle, and reacted at 180° C. for 24 hours. Centrifuge the obtained solid, wash it with deionized water and ethanol alternately for more than three times, dry it, and grind it. Mix 100 mg of the obtained powder with 40 mL of 1 uM CYP119 enzyme solution, stir at 10°C for 12 h, centrifuge, and wash to obtain the product.

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Abstract

The present invention relates to a kind of utilization TiO 2 ‑Phenanthroline-loaded CYP119 enzyme photocatalytic oxidation degradation method of methyl orange, the obtained material can effectively degrade the organic pollutant methyl orange. The method comprises: after uniformly mixing n-butyl titanate solution and o-phenanthroline solution, transferring to a hydrothermal tank for reaction. After the reaction, the sample is separated, washed, dried and ground, and mixed with CYP119 enzyme to obtain the product. It is characterized by the use of organic phenanthroline modified TiO 2 As a carrier of CYP119 enzyme, the obtained material has efficient photocatalytic oxidation performance.

Description

technical field [0001] The invention relates to the technical field of photocatalysis and biocatalysis, in particular to a method using TiO 2 - A method for the photocatalytic oxidation degradation of methyl orange loaded with o-phenanthroline and CYP119 enzyme. Background technique [0002] The CYP119 enzyme is a cytochrome P450 enzyme with exceptional stability and tolerance. Its thermal denaturation temperature is as high as 90°C, and it can withstand high pressure and extreme pH conditions above 200 MPa. It is an excellent biocatalyst and an ideal target for protein engineering. It has extremely important roles in the fields of biopharmaceuticals and biosynthesis. Value. Recent studies have shown that it has a special photoactivation mechanism, which provides a basis for the construction of a photocatalytic system based on CYP119 enzymes, making it possible to replace complex and expensive auxiliary enzyme systems with simple and cheap photocatalytic systems, which wil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N11/00
CPCC02F3/342C02F2305/10C12N11/00
Inventor 蒋华麟陈萍华刘军厉梦琳田磊张为波
Owner NANCHANG HANGKONG UNIVERSITY
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