DNA database establishing method for high-throughput sequencing

Abstract

The invention provides a DNA database establishing method for high-throughput sequencing, and particularly discloses a cfDNA database establishing method. On the basis of a standard database establishing method of Ion AmpliSeq Library Kit 2.0, the agent use amount and the PCR amplification conditions are optimized, and therefore reaction consumption is reduced, amplification specificity is higher, and the initial DNA amount is lower and even lower than 1 ng.

Description

technical field [0001] The invention relates to a method for building a DNA library for high-throughput sequencing, in particular to a method for building a cfDNA library for high-throughput sequencing. Background technique [0002] Malignant tumor is one of the major diseases threatening human health, and it is also the main cause of global morbidity and death. According to the data released by the World Health Organization, there were approximately 14 million new cancer cases and 8.2 million cancer-related deaths in 2012. According to the 2012 data released by the National Cancer Registry Center, there are about 3.5 million new cases in China every year, accounting for about one-fifth of the global incidence; the number of cancer deaths is about 2.5 million, accounting for about one-quarter of the global cancer deaths. Although my country has made great progress in tumor surgery, chemical drug treatment, radiotherapy and biotherapy technology, the population base makes my...

AI Technical Summary

Problems solved by technology

However, when performing high-throughput sequencing on plasma cfDNA, due to the low cfDNA content in plasma, the amount of cfDNA that can be obtained is very small, especially when the amount of blood available is small, the extracted cfDNA often cannot reach 10ng. Therefore, it is necessary to Develop a method that utilizes less blood for banking

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