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A method for preparing microsphere scaffolds using 3D reverse modeling technology

A microsphere and 3D technology, which is applied in the field of preparation of microsphere scaffolds for bone repair, can solve the problems of low porosity and pore connectivity, limited preparation conditions, and inability to prepare composite scaffolds, etc., to achieve high porosity and pore connectivity rate effect

Active Publication Date: 2018-12-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Among the existing technologies, the preparation methods of scaffolds are mainly sintering method and bonding method, etc. The scaffolds prepared by this method have good mechanical strength, but generally have the defects of low porosity and pore connectivity.
Scaffolds prepared by 3D printing technology have high porosity and pore connectivity, but limited by the preparation conditions, composite scaffolds cannot be prepared to a certain extent.

Method used

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  • A method for preparing microsphere scaffolds using 3D reverse modeling technology
  • A method for preparing microsphere scaffolds using 3D reverse modeling technology
  • A method for preparing microsphere scaffolds using 3D reverse modeling technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Dissolve 1g PLGA (polylactic-glycolic acid) in 16ml CH 2 Cl 2 , ultrasonically vibrated for 15 min until PLGA was completely dissolved.

[0038] 2) Weigh 0.1g of HMS (composite hexagonal mesoporous silicon) and 0.1g of β-TCP (β-type tricalcium phosphate) into the liquid obtained in step 1), and ultrasonically vibrate for 10 minutes to disperse evenly to obtain a dispersion .

[0039] 3) Weigh 3g of PVA (polyvinyl alcohol) and dissolve it in 300ml of deionized water, add the dispersion obtained in step 2) after the PVA is completely dissolved, and keep stirring for 8 hours.

[0040] 4) After the stirring is stopped, the liquid is centrifuged to take out the microspheres at the bottom, washed with deionized water for 5 times, and then freeze-dried the taken out microspheres to remove the water in the microspheres to obtain PLGA / HMS / β-TCP microspheres. ball.

Embodiment 2

[0042] 1) Dissolve 0.8g PLGA (polylactic glycolic acid) in 16ml CH 2 Cl 2 , ultrasonically vibrated for 15 min until PLGA was completely dissolved.

[0043] 2) Weigh 0.1g of HMS (composite hexagonal mesoporous silicon) and 0.1g of β-TCP (β-type tricalcium phosphate) into the liquid obtained in step 1), and ultrasonically vibrate for 10 minutes to disperse evenly to obtain a dispersion .

[0044] 3) Weigh 3g of PVA (polyvinyl alcohol) and dissolve it in 300ml of deionized water, add the dispersion obtained in step 2) after the PVA is completely dissolved, and keep stirring for 8 hours.

[0045] 4) After the stirring is stopped, the liquid is centrifuged to take out the microspheres at the bottom, washed with deionized water for 5 times, and then freeze-dried the taken out microspheres to remove the water in the microspheres to obtain PLGA / HMS / β-TCP microspheres. ball.

Embodiment 3

[0047] 1) Dissolve 1.2g PLGA (polylactic glycolic acid) in 16ml CH 2 Cl 2 , ultrasonically vibrated for 15 min until PLGA was completely dissolved.

[0048] 2) Weigh 0.1g of HMS (composite hexagonal mesoporous silicon) and 0.1g of β-TCP (β-type tricalcium phosphate) into the liquid obtained in step 1), and ultrasonically vibrate for 10 minutes to disperse evenly to obtain a dispersion .

[0049] 3) Weigh 3g of PVA (polyvinyl alcohol) and dissolve it in 300ml of deionized water, add the dispersion obtained in step 2) after the PVA is completely dissolved, and keep stirring for 8 hours.

[0050] 4) After the stirring is stopped, the liquid is centrifuged to take out the microspheres at the bottom, washed with deionized water for 5 times, and then freeze-dried the taken out microspheres to remove the water in the microspheres to obtain PLGA / HMS / β-TCP microspheres. ball.

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Abstract

The invention discloses a method for preparing a microsphere scaffold by utilizing a 3D reverse technology. A paraffin scaffold model is printed out through a 3D printing technology, microspheres are added to the scaffold model and fill the gaps of a scaffold; the scaffold is sintered to enable the microspheres to be mutually bonded to form the scaffold, the whole scaffold is immersed into gasoline to dissolve the paraffin scaffold, the scaffold is cleaned with ethanol and is dried at room temperature, and finally the microsphere scaffold is obtained. The microsphere scaffold obtained by means of the method has higher porosity and pore connectivity rate, and the method also overcomes the severe requirements of the 3D printing technology to the viscosity and other natures of a printing material.

Description

technical field [0001] The invention relates to the field of preparation of microsphere scaffolds for bone repair, in particular to a method for preparing microsphere scaffolds using 3D reverse modeling technology. Background technique [0002] In some areas, microspheres for bone repair can be made into shaped scaffolds. In the field of bone tissue repair, microsphere scaffolds can provide certain mechanical strength. The microsphere scaffold has a certain porosity, which is conducive to the flow of liquid and also provides attachment sites for cells. In cell culture experiments, the drug-loaded microsphere scaffolds can greatly increase the survival rate of cells, while also reducing the adhesion of dead cells. [0003] Among the existing technologies, the preparation methods of scaffolds are mainly sintering method and bonding method. The scaffolds prepared by this method have good mechanical strength, but generally have the defects of low porosity and pore connectivity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/02A61L27/12A61L27/16A61L27/56
CPCA61L27/02A61L27/12A61L27/16A61L27/18A61L27/56A61L2300/112A61L2300/412A61L2430/02C08L67/04C08L29/04
Inventor 魏坤梁猛
Owner SOUTH CHINA UNIV OF TECH