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Liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in dry blood sample and kit

A hydroxyvitamin and tandem mass spectrometry technology is applied in the field of 25 hydroxyvitamin D detection, and achieves the effects of simple pretreatment, improved detection accuracy, and batch processing.

Inactive Publication Date: 2016-06-08
北京洛奇医学检验实验室股份有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to solve some problems existing in the existing 25-hydroxyvitamin D detection method technology, and to provide a liquid chromatography tandem mass spectrometry detection method and kit for 25-hydroxyvitamin D in dried blood samples

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  • Liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in dry blood sample and kit
  • Liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in dry blood sample and kit
  • Liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in dry blood sample and kit

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[0075] 1. Whole blood matrix preparation

[0076] The whole blood was centrifuged to remove the upper layer of plasma, and the blood cells were reserved for later use. The blood cells were washed 3 times with 0.9% normal saline, and then mixed with normal saline 1:1 to obtain the processed whole blood.

[0077] Dried blood spot standard curve sample and quality control dried blood spot sample (QC blood film) preparation

[0078] Preparation of the dried blood spot standard curve sample: Take 2ml of processed whole blood and mix with 4μl, 10μl, 20μl, 50μl, 100μl, 200μl of 25-OHD with a concentration of 1μg / ml to obtain 2-100ng / ml ml of dried blood spot standard curve sample, respectively take 75 μl of the prepared sample and drop it on 903 filter paper to dry it.

[0079] Preparation of the quality control dried blood spot sample: Take 2ml of processed whole blood and mix them with 16μl, 40μl, and 80μl of 25-OHD with a concentration of 1μg / ml respectively to obtain 8ng / ml (Q1...

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Abstract

The invention relates to a liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in a dry blood sample. The pretreatment technique mainly comprises the processes of sample pretreatment, deriving and redissolving, and meanwhile, the multi-reaction monitoring MRM scanning mode is adopted. According to the method, the high-flux liquid chromatogram tandem mass spectrum detection method for 25-hydroxy vitamin D in the dry blood sample can be realized; the problems of large demanded quantity of samples and inconvenience in sampling and transporting of the traditional liquid chromatogram tandem mass spectrum method are solved; through the improvement for the detection technique, the detection for the 25-hydroxy vitamin D in the dry blood sample is realized, the detector can voluntarily collect at home, the demanded quantity of samples is small and the sampling and transporting are convenient.

Description

technical field [0001] The invention relates to the technical field of 25-hydroxyvitamin D detection, in particular to an improved liquid chromatography tandem mass spectrometry method for detecting 25-hydroxyvitamin D. Background technique [0002] In the past decade, a large number of studies have shown that vitamin D plays an important role in health and disease (Holick MF. Deficiency of sunlight and vitamin D. BMJ 2008; 336: 1318-9. Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. AmJ Clin Nutr 2008; 87: 1080S-6S.). Vitamin D is not only closely related to rickets, osteoporosis, etc., but also related to cancer, hypertension, diabetes, multiple sclerosis, depression, etc. (HolickMF.VitaminDdeficiency.NEnglJMed2007; 357:266–81.). Studies have shown that vitamin D levels in childhood are associated with many disorders in late childhood or adulthood (McGrathJ. 新生儿股骨长度有关(MorleyR,CarlinJB,PascoJA,WarkJD.Maternal25-hydroxyvitaminDandparat...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062G01N2030/067
Inventor 何健郭玉梅宋晓涛
Owner 北京洛奇医学检验实验室股份有限公司
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