Double-strand oligonucleotide-epsilon-polylysine compound with immune regulation function and preparation and use method of double-strand oligonucleotide-epsilon-polylysine compound

A double-stranded polynucleotide and polylysine technology, applied in biochemical equipment and methods, medical preparations containing active ingredients, microorganisms, etc., can solve problems such as antibiotic residues, and achieve strong resistance to biological enzymatic degradation , Improving immune regulation activity, simple preparation process

Active Publication Date: 2016-06-15
辽科生物(沈阳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0047] In 1969, the Chinese Academy of Sciences collaborated with relevant units to creatively use a new formulation, and developed a new polymuscular cell complex formulation, that is, adding kanamycin sulfate containing polyamide positive ions on the basis of PIC to Protect the double-strand structure from rapid hydrolysis by nucleases

Method used

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  • Double-strand oligonucleotide-epsilon-polylysine compound with immune regulation function and preparation and use method of double-strand oligonucleotide-epsilon-polylysine compound
  • Double-strand oligonucleotide-epsilon-polylysine compound with immune regulation function and preparation and use method of double-strand oligonucleotide-epsilon-polylysine compound
  • Double-strand oligonucleotide-epsilon-polylysine compound with immune regulation function and preparation and use method of double-strand oligonucleotide-epsilon-polylysine compound

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0091] Example 1

[0092] a. Preparation of 0.1mol / L calcium chloride solution:

[0093] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize it through a filter membrane with a 0.2 μm pore size to prepare a 0.1 mol / L calcium chloride solution.

[0094] b. Preparation of 0.01mol / L PBS solution (pH=7.6):

[0095] Weigh 0.124 g of disodium hydrogen phosphate, 0.018 g of sodium dihydrogen phosphate monohydrate, and 0.808 g of sodium chloride, respectively, dissolve them in 100 ml of water for injection after mixing, and filter and sterilize them through a filter membrane with a 0.2 μm pore size to make 0.01 mol / L. PBS solution (pH=7.6).

[0096] c. Preparation of 2mg / ml PolyIC intermediate solution:

[0097] Weigh 1.0088g of PolyI and 0.9912g of PolyC, respectively, add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, put it in a water bath at 45°C for 60 m...

Example Embodiment

[0104] Example 2

[0105] a. Preparation of 0.1mol / L calcium chloride solution:

[0106] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize it through a filter membrane with a 0.2 μm pore size to prepare a 0.1 mol / L calcium chloride solution.

[0107] b. Preparation of 0.01mol / L PBS solution (pH=7.6):

[0108] Weigh 0.124 g of disodium hydrogen phosphate, 0.018 g of sodium dihydrogen phosphate monohydrate, and 0.808 g of sodium chloride, respectively, dissolve them in 100 ml of water for injection after mixing, and filter and sterilize them through a filter membrane with a 0.2 μm pore size to make 0.01 mol / L. PBS solution (pH=7.6).

[0109] c. Preparation of bursal virus RNA solution of 2 mg / ml:

[0110] The bursal virus RNA was dissolved in PBS buffer, filtered and sterilized through a 0.45 μm pore size filter to prepare a 2 mg / ml solution for later use.

[0111] d. Preparation of 10mg / ml ε-polylysine soluti...

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PUM

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Abstract

The invention provides a double-strand oligonucleotide-epsilon-polylysine compound with an immune regulation function and a preparation and use method of the double-strand oligonucleotide-epsilon-polylysine compound and also provides immunogen composition with functions of triggering immunoenhancement reaction and changing the immunoreaction type and a preparation and use method of the immunogen composition. The immunogen composition comprises the double-strand oligonucleotide-epsilon-polylysine compound and an antigen. The compound contains a compound formed through composition of double-stranded polynucleotide, a cation epsilon-polylysine and metal cations. The compound has the pharmacological activity of immunoregulation. The preparation process of the compound is simple. The compound has good water solubility and a bio-enzyme degradation resisting function. The immunogen composition can trigger the immunoenhancement reaction and change the immunoreaction type.

Description

technical field [0001] The present invention relates to the field of medicine, specifically, the present invention provides a double-stranded polynucleotide-ε-polylysine complex with immune regulation function and its preparation and use method. The present invention also provides an immunogen composition capable of triggering and enhancing immune response and changing the type of immune response and its preparation and use method. Background technique [0002] 1. Double-stranded polynucleotide: Double-stranded polynucleotide or its derivatives have various pharmacological activities, and the source is natural extraction or artificial synthesis. Artificially synthesized double-stranded polynucleotides such as polyinocytic (polyI:C) can induce the production of interferon in the body, protect cells from virus infection, and have curative effects such as preventing and treating viral diseases; naturally extracted double-stranded polynucleotides such as Viral double-stranded R...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/12
CPCA61K39/12A61K39/39A61K2039/55511A61K2039/55583C12N2760/20134
Inventor 苏文全
Owner 辽科生物(沈阳)有限公司
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