Application of Cicadae Cordyceps Polysaccharide in Preparation of Chicken Immunity Enhancement Reagent

A technology for enhancing immunity, such as antiviral agents, biochemical equipment and methods, and medical preparations containing active ingredients, can solve problems such as difficulty in generating immune responses, and inability to completely control infectious diseases. Enhanced cellular immunity and humoral immunity, no hidden dangers of drug residues, and improved efficacy

Active Publication Date: 2019-06-07
ZHEJIANG SUB TROPICS CROP INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the farms have carried out strict vaccination according to the immunization procedures, in many cases, it is still difficult for the animals to produce an effective immune response after immunization, so that the infectious diseases cannot be completely controlled

Method used

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  • Application of Cicadae Cordyceps Polysaccharide in Preparation of Chicken Immunity Enhancement Reagent
  • Application of Cicadae Cordyceps Polysaccharide in Preparation of Chicken Immunity Enhancement Reagent
  • Application of Cicadae Cordyceps Polysaccharide in Preparation of Chicken Immunity Enhancement Reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Separation and Purification of a Single Component of Cicadaea Cordyceps Polysaccharide

[0020] 1.1 Extraction and preparation of polysaccharides from Cordyceps cicadae

[0021] Grind Cordyceps cicadae and pass through a 60-80 mesh sieve; add 10 times the volume of 50% ethanol to the crushed raw material, carry out hot reflux auxiliary extraction, filter, and repeat the above steps for the filter residue, repeat 1-3 times; combine multiple filtrates . Concentrate under reduced pressure at 70°C to one-tenth of the original volume; add 3 times the volume of 95% ethanol to the above concentrated solution while stirring, let it stand for more than 4 hours, and collect the precipitate by centrifugation; wash the precipitate with 3 times the volume of ethanol, centrifuge, and vacuum Dried to obtain a dark brown powdery Cordyceps polysaccharide product.

[0022] 1.2 Separation and purification of polysaccharides from Cordyceps cicadae

[0023] In the last step of...

Embodiment 2

[0024] Embodiment 2. The influence of the polysaccharide of Cicadae sinensis on chicken immunity

[0025] 2.1 Materials and methods

[0026] 2.1.1 Test animals

[0027] The chicks were reared in the poultry breeding and hatching room of Wenzhou Vocational College of Science and Technology. They were fed and drank ad libitum. Keep the feeding environment conditions of each group consistent, disinfect regularly, keep the sanitation in the house, and follow the routine feeding management.

[0028] 2.1.2 Drugs and diagnostic reagents

[0029] Cordyceps cicadae polysaccharide extract, astragalus polysaccharide (manufactured by Guangdong Tianbao Biopharmaceutical Co., Ltd.), Newcastle disease standard positive serum, negative serum and Newcastle disease virus hemagglutination inhibitory antigen (China Veterinary Drug Supervision 20140301), Newcastle disease IV series live vaccine ( Lasota strain) (virus vaccine), Newcastle disease oil emulsion inactivated vaccine (produced by Zhe...

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Abstract

The invention relates to the use of the polysaccharide of Cordyceps cicadae in the preparation of chicken immune enhancing reagents, and belongs to the field of livestock and poultry immune adjuvants. The polysaccharide is obtained from the fruiting bodies of the cicadae or the culture medium by heat reflux and then separated and purified by a ceramic membrane. The process of the present invention is simple, and it is proved by immunization experiments that the Cicadae sinensis polysaccharide immunopotentiator can significantly enhance the immune function of the chicken body; when combined with the Newcastle disease vaccine to immunize chicks, the immune response of the vaccine can be significantly improved, and the immune effect lasts for a long time. The preparation has no problems such as drug residues, toxic side effects, etc., and is a green, efficient and stable chicken immune enhancer.

Description

technical field [0001] The invention relates to the use of the polysaccharide of Cordyceps cicadae in the preparation of chicken immune enhancing reagents, and belongs to the field of livestock and poultry immune adjuvants. Background technique [0002] Infectious diseases, especially viral infectious diseases, have caused serious losses to animal husbandry all over the world. Vaccination is currently the most effective means of controlling infectious diseases. However, although the farms have carried out strict vaccination according to the immunization procedures, in many cases, it is still difficult for the animals to produce an effective immune response after immunization, so that the infectious diseases cannot be completely controlled. If the vaccine is injected at the same time, the appropriate immune enhancer can be used to assist the vaccine to produce a better immune response and improve its immune efficacy, thereby improving the effect of vaccine immunity. The appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/17A61K36/066A61P31/14A61K35/64
CPCA61K35/63A61K36/066A61K39/12A61K39/39A61K2039/55583C12N2760/18134
Inventor 柴一秋刘素贞朱碧纯厉晓腊
Owner ZHEJIANG SUB TROPICS CROP INST
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