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Serum markers for preoperatively detecting liver cancer microvascular invasion

A technology of markers and microvessels, applied in the field of serum markers, can solve the problems of lack of efficient and accurate methods, ineffective identification of proteins, and poor sensitivity

Active Publication Date: 2016-06-22
李江涛
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  • Abstract
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  • Application Information

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Problems solved by technology

In recent years, more and more scholars have studied MVI-related markers from the level of molecular biology, but the specificity and accuracy of these markers are not ideal, and they are far from clinical diagnosis
In the past, the screening method for liver cancer antigens was mainly based on two-dimensional electrophoresis-mass spectrometry analysis technology, but this technology has some shortcomings: 1. The specificity of identifying antigens by two-dimensional electrophoresis between normal liver tissue and liver cancer tissue and mass spectrometry analysis is insufficient;2. . There are large differences between individuals, and liver cancer has multiple stages, so the result deviation is large; 3. Some important proteins cannot be effectively identified, and the sensitivity is poor
At present, there is still a lack of an efficient and accurate method for the preoperative detection of liver cancer MVI.

Method used

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  • Serum markers for preoperatively detecting liver cancer microvascular invasion
  • Serum markers for preoperatively detecting liver cancer microvascular invasion
  • Serum markers for preoperatively detecting liver cancer microvascular invasion

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Embodiment Construction

[0012] The screening and reverse verification process of the serum markers of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0013] The present invention collects and sorts out 160 liver cancer tissue sample banks, including preoperative peripheral blood, postoperative liver cancer tissue, adjacent normal tissue, and related clinical case data. refer to figure 1 The flow shown is for reverse serum-proteomics experimental research.

[0014] 1. Samples to be collected, each case contains:

[0015] 1) Tumor tissue paraffin block × 1;

[0016] 2) Fresh specimens of tumor tissue x 1 are stored in a -80°C refrigerator;

[0017] 3) Paraffin block of normal liver tissue adjacent to the tumor × 1;

[0018] 4) A fresh specimen of normal liver tissue adjacent to the tumor x 1 is stored in a -80°C refrigerator;

[0019] 5) Store the plasma supernatant × 1 in a -80°C refrigerator after two centrifugations;

[0020] 6) After ce...

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Abstract

The invention discloses serum markers for preoperatively detecting liver cancer microvascular invasion. A reverse serum-protein omics technology is adopted to in vivo study associated antigens and antibodies of liver cancer MVI for the first time, the serum markers for preoperatively detecting liver cancer microvascular invasion are determined, and an effective detection means is provided for clinical preoperative early diagnosis of MVI.

Description

technical field [0001] The invention relates to a serum marker, in particular to a serum marker used for preoperative detection of liver cancer microvessel invasion. Background technique [0002] Microvascular invasion (MVI) of hepatocellular carcinoma refers to the observation under the microscope that cancer cell nests can be seen in the vascular lumen lined by endothelial cells. Occult metastases produced by liver cancer cells invading microvessels are one of the important reasons for postoperative recurrence of liver cancer. Literature has confirmed that MVI is an independent indicator for predicting recurrence and evaluating prognosis after liver cancer resection. American Joint Committee on Cancer (American Joint Committee on Cancer, AJCC) guidelines have taken MVI as one of the important factors for TMN staging of liver cancer. Currently, the diagnosis of MVI is based on pathological examination of postoperative or biopsy needle specimens. At the imaging level, some...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/573G01N33/57438G01N33/57484
Inventor 李江涛金赟于源泉周佳乐钱程明杨俊杰张小小
Owner 李江涛
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