Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds
A technology of Melaleuca alternifolia and rooting culture, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of limited rooting rate of tissue culture technology, and save the purchase cost and the labor cost of cleaning. , the development of the survival rate, the effect of reducing production costs
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Embodiment 1
[0028] Carry out rooting cultivation according to the method of the present invention in March in Nanning, concrete steps are as follows:
[0029] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;
[0030] (2) Preparation of rooting medium: The medium formula adopts 1 / 2 modified WPM medium plus IBA0.5mg / L, ABT6 No. 0.6mg / L, sucrose 15000mg / L, agar 6.0g / L;
[0031] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use ;
[0032] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches and leaves, ...
Embodiment 2
[0041] Carry out rooting cultivation according to the method of the present invention in September in Nanning, concrete steps are as follows:
[0042] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;
[0043] (2) Preparation of rooting medium: The medium formula adopts 4 / 5 modified WPM medium plus IBA0.7mg / L, ABT6 No. 0.2mg / L, sucrose 15000mg / L, and agar 6.0g / L.
[0044] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use .
[0045] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches an...
Embodiment 3
[0054] Carry out rooting cultivation according to the method of the present invention in November in Nanning, concrete steps are as follows:
[0055] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;
[0056] (2) Preparation of rooting medium: The medium formula adopts 1 / 2 modified B5 medium plus IBA0.05mg / L, ABT6 No. 1.0mg / L, sucrose 15000mg / L, and agar 6.0g / L.
[0057] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use .
[0058] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches and...
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