Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds

A technology of Melaleuca alternifolia and rooting culture, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of limited rooting rate of tissue culture technology, and save the purchase cost and the labor cost of cleaning. , the development of the survival rate, the effect of reducing production costs

Active Publication Date: 2016-06-29
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among the essential oil components of Melaleuca alternifolia, the most important active ingredient is 4-terpineol. After years of breeding by researchers, several 4-terpineol-type Alternaria alternifolia with higher terpineol content have been selected. Melaleuca is an excellent variety, but the higher terpineol component shows a negative impact on root germination during tissue culture, which limits the rooting rate of existing tissue culture techniques

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0028] Carry out rooting cultivation according to the method of the present invention in March in Nanning, concrete steps are as follows:

[0029] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;

[0030] (2) Preparation of rooting medium: The medium formula adopts 1 / 2 modified WPM medium plus IBA0.5mg / L, ABT6 No. 0.6mg / L, sucrose 15000mg / L, agar 6.0g / L;

[0031] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use ;

[0032] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches and leaves, ...

Embodiment 2

[0041] Carry out rooting cultivation according to the method of the present invention in September in Nanning, concrete steps are as follows:

[0042] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;

[0043] (2) Preparation of rooting medium: The medium formula adopts 4 / 5 modified WPM medium plus IBA0.7mg / L, ABT6 No. 0.2mg / L, sucrose 15000mg / L, and agar 6.0g / L.

[0044] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use .

[0045] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches an...

Embodiment 3

[0054] Carry out rooting cultivation according to the method of the present invention in November in Nanning, concrete steps are as follows:

[0055] (1) Cultivation of strong seedlings of subcultured buds: use conventional subculture medium for proliferation and culture of subcultured buds; when the length of a single bud of subcultured buds reaches 1.0 cm, increase the light to promote maturity of single buds;

[0056] (2) Preparation of rooting medium: The medium formula adopts 1 / 2 modified B5 medium plus IBA0.05mg / L, ABT6 No. 1.0mg / L, sucrose 15000mg / L, and agar 6.0g / L.

[0057] In the special laboratory for preparation of culture medium, the test table and the surrounding operating space are sprayed with 75% alcohol to disinfect; other utensils in the packaging process are soaked and disinfected with 0.3% bromogeramine for 15 minutes, and the polyethylene plastic cup is for one-time use .

[0058] Take 250g of annual 4-terpineol-type Melaleuca alternifolia branches and...

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Abstract

The invention discloses an open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds.By optimally regulating the composition and ratio of a culture medium, a culture medium formula capable of promoting rooting of the 4-terpilenol melaleuca alternefolia tissue culture subculture buds is obtained by means of screening, a proper bacteriostatic agent is added assisted by appropriate temperature and illumination, and the open rooting culture method for the 4-terpilenol melaleuca alternefolia tissue culture subculture buds is established.By means of the method, finished products produced at the rooting culture stage can be greatly reduced, and the contamination rate of open rooting culture is effectively controlled; the rooting culture period is short, a root system is germinated 6-8 days after rooting culture, and the rooting rate reaches 97.8%-99.5%; according to statistics conducted after culture is continued for 15 days, the rooting rate can reach 100%, the contamination rate is lower than 10%, the root system of seedlings is robust and developed, the survival rate of transplanting is high and reaches 97.4%-98.8%, and good economic benefits, social benefits and ecological benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of plants, and relates to a method for rooting tissue-cultured seedlings, in particular to an open-type rooting method for 4-terpineol-type Melaleuca alternifolia tissue-cultured seedlings. Background technique [0002] Melaleuca alternifolia ( Melaleuca ahemifolia ) is a spice plant of the Myrtaceae Melaleuca genus, introduced from Australia to southern my country in the early 1990s, and is currently planted in Guangxi, Guangdong, Yunnan and Fujian provinces. The essential oil obtained by steam distillation of the stems and leaves of Melaleuca alternifolia is also called tea tree oil. It is not only a natural spice, but also has a strong bactericidal effect and has a high economic value. [0003] Most of the seeds of Melaleuca alternifolia are aborted, the germination rate is low, and there are large differences between species. Therefore, the current breeding of Melaleuca alte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00Y02A40/22Y02P60/40
Inventor 陈博雯刘海龙肖玉菲覃子海张烨张晓宁
Owner GUANGXI FORESTRY RES INST
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