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Application of borneol in promoting poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation

A technology of doxorubicin and chloride channel, which is applied in the field of medicine to achieve the effect of strengthening the anti-tumor effect

Inactive Publication Date: 2016-07-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0028] Is borneol's promotion of drug uptake by cells related to chloride channels? There are few reports

Method used

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  • Application of borneol in promoting poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation
  • Application of borneol in promoting poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation
  • Application of borneol in promoting poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation

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Experimental program
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Embodiment 1

[0070] 1. Materials and methods

[0071] 1.1 Materials

[0072] 1.1.1 Main instruments and equipment

[0073] Table 1 Main instruments and equipment

[0074]

[0075]

[0076] 1.1.2 Cells and Drugs

[0077] The cells studied in this experiment are human poorly differentiated nasopharyngeal carcinoma epithelial cells (CNE-2Z), donated by Professor Tang Weiping from the Department of Pathology, Guangdong Medical College.

[0078] 1.1.3 Main Reagents and Drugs

[0079] Reagents and medicines for culturing cells

[0080] 1. Cell culture medium: purchase RPMI1640 powder containing L-glutamin from GIBCO, prepare it with deionized water according to the instructions, filter it with a 0.22 μm filter membrane, and store it in the refrigerator at 4°C.

[0081] 2. Fetal bovine serum: purchased from GIBCO, aliquoted to 10mL / bottle, and stored in a refrigerator at -20°C.

[0082] 3. Penicillin streptomycin: which contains 1×10 4IU / mL penicillin and 1×10 4 μg / mL streptomycin...

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Abstract

The invention discloses an application of borneol in promoting a poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation. With the human poorly differentiated nasopharyngeal cell (CNE-2Z) as a research object, the conclusions are explored from the angle of the chloride channel as follows: (1) the chloride channel can be an action target point of the borneol in promotion of adriamycin transportation across a CNE-2Z cytomembrane; (2) the borneol can activate a CNE-2Z cell chloride current, and the current has volume sensitivity; (3) the ClC-3 chloride channel plays an important role in promotion of adriamycin transportation across the CNE-2Z cytomembrane by the borneol; and (4) the anti-tumor effect of the adriamycin can be obviously improved by combination of the borneol and the adriamycin. The mechanism of the borneol in promotion of drug uptake through the cytomembrane is expected to be diluted from the angle of the chloride channel by discussing the relationship between the chloride channel and the drugs taken by the cell in different states; and a new theoretical basis is provided for the borneol in clinical application.

Description

technical field [0001] The invention belongs to the field of medicine, and particularly relates to the application of borneol to promote the uptake of doxorubicin by poorly differentiated nasopharyngeal carcinoma cells through the mediation of chloride channels. Background technique [0002] Cancer is now an important cause of endangering human health and causing human death. The incidence of cancer presents an increasing trend year by year and a younger trend. But so far, there is still no means of a complete cure. Although the treatment methods are constantly being updated, chemotherapy, as a classic anti-tumor treatment, still plays a pivotal role. The drug resistance of tumor cells is the biggest problem in chemotherapy and chemotherapy. One of the main reasons for drug resistance is the reduction of drug uptake by cells or the increase of drug efflux from cells, resulting in a decrease in intracellular drug concentration. [0003] There are two main methods to promo...

Claims

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Application Information

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IPC IPC(8): A61K31/045A61K31/704A61P35/00
CPCA61K31/045A61K31/704A61K2300/00
Inventor 陈丽新王立伟朱林燕魏燕孟龙罗海张海峰叶东王海波
Owner JINAN UNIVERSITY
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