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A preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine

A polyethyleneimine, fluorescent polymer technology, applied in the field of nanomaterials, can solve the problems of complicated synthesis process, high cost, poor environmental protection, etc., and achieve the effects of excellent fluorescence performance, strong stability and good stability

Inactive Publication Date: 2018-05-25
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems of cumbersome synthesis process, high cost and poor environmental protection of fluorescent materials existing in the prior art, the present invention provides a preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine, which directly uses polyethyleneimine as One-step Synthesis of Fluorescent Polymer Nanoparticles by Acylation Reaction of Matrix and Ascorbic Acid

Method used

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  • A preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine
  • A preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine
  • A preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine

Examples

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Effect test

Embodiment 1

[0033] 1.1 Weigh 0.7046 g of polyethyleneimine (molecular weight: 600) into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare A solution;

[0034] 1.2 Weigh 0.3523 g of ascorbic acid into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0035] 1.3 Take 100 μL of solution B, add 50 μL of solution A to it, and then add 350 μL of ultrapure water, shake and mix evenly to obtain the fluorescent polymer nanoparticle solution;

[0036] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.1 nm, and it takes 14 days to stabilize, and the stable period is at least 3 months.

Embodiment 2

[0038] 2.1 Weigh 0.7046 g of polyethyleneimine (molecular weight: 1300) into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare A solution;

[0039] 2.2 Weigh 0.3523 g of ascorbic acid into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0040] 2.3 Take 100 μL of solution B, add 100 μL of solution A to it, and then add 300 μL of ultrapure water, shake and mix evenly to obtain the fluorescent polymer nanoparticle solution;

[0041] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.2 nm, and it takes 13 days to stabilize, and the stable period is at least 3 months.

Embodiment 3

[0043] 3.1 Weigh 0.7046 g of polyethyleneimine (molecular weight: 1800) into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare A solution;

[0044] 3.2 Weigh 0.3523 g of ascorbic acid into a 4 mL plastic centrifuge tube, add 2 mL of ultrapure water into it, shake and mix evenly to prepare B solution;

[0045] 3.3 Take 100 μL of solution B, add 150 μL of solution A to it, and then add 250 μL of ultrapure water, shake and mix evenly to obtain the fluorescent polymer nanoparticle solution;

[0046] The particle size of the fluorescent polymer nanoparticles prepared in this example is 9.2 nm, and it takes 13 days to stabilize, and the stable period is at least 3 months.

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Abstract

The invention discloses a preparation method for synthesizing fluorescent polymer nanoparticles on the basis of polyethyleneimine. The preparation method is implemented in steps as follows: solutions are prepared from polyethyleneimine and ascorbic acid respectively, mixed and then subjected to an acylation reaction, and the fluorescent polymer nanoparticles are prepared through one-step synthesis. The provided preparation method of the fluorescent polymer nanoparticles is simple and easy to operate, and the fluorescent polymer nanoparticles have good water solubility, have high fluorescence in the range of 350-370 nm and excellent stability, contain a large quantity of active groups allowed to be modified, can be used for the field of microimaging, biological probes, recognition and the like and have excellent application prospect.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a preparation method for synthesizing fluorescent polymer nanoparticles based on polyethyleneimine. Background technique [0002] Polyethyleneimine (PEI) is a water-soluble high-density cationic polymer, a colorless or pale yellow viscous liquid, hygroscopic, soluble in water and ethanol, and insoluble in benzene. It has good water solubility, high cationicity, high reactivity and good gene transfection. In the prior art, the fluorescent nanomaterials synthesized by polyethyleneimine have complex synthesis steps and often contain noble metals, heavy metals, etc., such as gold, silver, sulfur, cadmium, zinc, etc., so that the related synthesis of the prepared fluorescent nanomaterials The matrix is ​​expensive, poor in environmental protection, and poor in biocompatibility. The molecular structural formula of polyethyleneimine is as follows: [0003] [0004] T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/04C09K11/00
CPCC08G73/0206C09K11/00C09K2211/14
Inventor 曲斐王盛儒刘延群尤进茂
Owner QUFU NORMAL UNIV
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