Multiple RT-PCR detection kit for American type porcine reproductive and respiratory syndrome virus and porcine parvovirus

Abstract

The invention provides a multiple RT-PCR detection kit for American type porcine reproductive and respiratory syndrome virus and porcine parvovirus, comprising a primer pair 1 for amplifying a target gene of American type porcine reproductive and respiratory syndrome virus and a primer pair 2 for amplifying a target gene of porcine parvovirus. The invention also provides a use method of the detection kit. The detection kit provided by the invention is quick as compared with the classical method of virus isolation, single RT-PCR and serotype typing, and can conveniently identify and distinguish any one of the American type porcine reproductive and respiratory syndrome virus and porcine parvovirus once, so that timely and quick response can be made to epidemic disease conditions, regions and environments, and a correct method of disposition can be decided in the shortest time, which has a positive meaning for stopping or blocking infectious disease transmission; and simultaneously the detection kit is especially suitable for extraction of small samples and has broad market application prospects.

Description

technical field [0001] The invention relates to a livestock virus detection kit, in particular to a multiple RT-PCR detection kit for American type porcine reproductive and respiratory syndrome virus and porcine parvovirus. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV), with premature birth, abortion, stillbirth, weak piglets and Mummified fetuses, piglets and fattening pigs have respiratory diseases, reduced production performance, reduced feed remuneration, and increased mortality. At present, the threat of American PRSV is the most significant in China. [0003] Porcine parvovirus disease is a pig reproductive disorder caused by porcine parvovirus (Porcine parvovirus, PPV), and domestic pigs and wild boars of different ages and genders are susceptible. The source o...

AI Technical Summary

Problems solved by technology

[0004] Although a lot of research has been done on the etiology, serotyping, and diagnosis of PRRSV and PPV at home and abroad, because it is almost all over the world, the threat to pigs and related products around the world is well known, and the economic losses caused are at least above 100,000 per year. tens of billions of dollars

In particular, the two have similar clinical manifestations, and it is difficult to distinguish them clinically. At the same time, they may lack characteristic symptoms, which brings great difficulties to diagnosis and prevention.

Therefore, it is difficult to qualitatively rely on clinical diagnosis alone

[0005] In order to determine whether the above two diseases exist in a certain area, the method of only detecting animal serum antibodies is not completely reliable, and the existence of pathogens must also be confirmed through etiological testing. The general testing methods are biological experiments, serotype diagnostic techniques and Diagnostic techniques such as molecular biology techniques, but some of these methods have low sensitivity, some have low specificity, some have low detection rates, the detection cycle is too long, and these techniques are generally too expensive

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