46results about How to "Suitable for extraction" patented technology

The present invention relates to a method which utilizes fresh aromatic plants as materials and combines the microwave heating technology with the vacuum technology to prepare essential oil. The method includes the following steps: the tissue structure of aromatic plant material is broken by cutting and grinding; after microwave irradiation is utilized to heat the aromatic plant material to a certain temperature, pressure is rapidly reduced to intensely gasify the moisture in the material so that the histiocytes, oil vacuoles and gland cells of the aromatic plants are broken; an aromatic substance is released from the cells and quickly volatilize; and the effect of the intense gasification and spreading of the moisture in the fresh material can effectively promote and intensify the volatilization of the aromatic ingredient, so that the abstraction of the essential aromatic plant oil can be fulfilled in a short time. The processing temperature in the process of the abstraction of the essential plant oil is low, the oxidation of sensitive ingredients can be avoided, the stability of the ingredients of the essential aromatic plant oil can be kept to the max, the time of abstraction is short, the efficiency is high, energy can be saved, and industrialized production can be conveniently realized.

The invention discloses a purifying method of rice bran protein through composite enzyme, which comprises the following steps: allocating 10%-20% slurry with degreased rice bran and water; adjusting pH value to 5.0-6.5 and temperature to 40-50 deg.c; building up phytase, hemicellulase, cellulase and aspergillus protein oryzae with rate at 15.64í† 15.06í†60.78í†8.52; adding 2-4% composite enzyme; stirring 1.5-2h; centrifuging 20 min; obtaining the supernatant as rice bran protein solution; condensing in the vacuum; freezing; drying; obtaining the product with protein receiving rate to 90.5-92.7%.

This invention is rotating blade car type fluid power generator. The chain connects to three groups of rotating blade cars. Rotating blades are evenly distributed on circulate track. Two straight tracks and two half circular arc tracks are connected to form circulating track. Under the effect of fluid, rotating blade of rotating blade car is vertical to fluid flowing direction at the straight segment of track. Thus, fluid active force is strong. At another straight segment, under the effect of fluid, rotating blade of rotating blade car is parallel to fluid flowing direction. Thus, fluid active force is little. The three groups of rotating blade cars and rotating blades undertake circulating operation along track. The chain transfers power to power generator through driving train. The flow power driving train, which is applied underground, is composed of chain pump, pipe, accumulator and water turbine. It makes power generator can be assembled on water surface. The resistance type wind power machine is driven by chain.

The invention discloses a method for measuring spontaneous Brillouin scattering based on a super structured fiber grating filter, which inhibits coherent Rayleigh noise by the method which uses the distribution of a narrow line width to feedback a fiber optic laser and a wide band laser to obtain strength ratio of a Brillouin signal and a Rayleigh signal; the method comprises the following steps:firstly, the generated narrow line width pulsed light is fed back to a detected fiber after polarization control according to the length of the fiber; a back reflection signal enters a filter bank through a circulator port (4) and respectively regulates the adjustable super structured fiber grating filter, filters a Rayleigh signal to the largest extent, extracts a spontaneous Brillouin scatteringsignal, feeds the spontaneous Brillouin scattering signal into a highly sensitive photoelectricity conversion module and a signal processing module, and detects the intensity of the spontaneous Brillouin signal through data accumulation and wavelet-de-noising treatment; secondly, the pulsed light generated by the wide band laser passes through a rear-end module the same as in the first step to measure the Rayleigh scattering signal; and finally, the data measured at two times are processed on a computer to display the fiber temperature and stress, which solves the problem of measuring methodof the fiber spontaneous Brillouin signal.

The invention relates to a special efficient culture medium for necrotic fusobacterium and a preparation method thereof. The culture medium mainly comprises carbon source substances and nitrogen source substances. The invention is characterized in that the culture medium is prepared from 500 to 1000ml of basal culture solution 1, 40 to 50g of stomach peptone, 500 to 1000ml of basal culture solution 2, 500 to 1000ml of basal culture solution 3, 15 to 18g of Na2HPO4H.H2O, 30g of NaCl, 5 to 10g of protohemin, 0.5 to 1g of vitamin K, 2.5 to 5g of cysteine hydrochloride and 100 to 200g of glucose by regulating the pH value to 7.4-7.8. When the culture medium is used for cultivating necrotic fusobacterium, the necrotic fusobacterium grows quickly, the necrotic fusobacterium yield is large, and the cost is low. The special efficient culture medium for necrotic fusobacterium is suitable for vaccine production or extraction of leukotoxin.

The invention relates to an electric household appliance for culinary preparation for pressing foodstuffs, comprising a work chamber (40) accommodating a pressing screw (20) driven in rotation in a filter (30) comprising at least one filtering side portion (32a, 32b), the work chamber (40) having an outlet for discharging filtered foodstuffs and being arranged outside of the filter (30), the filter (30) comprising a side portion (34) devoid of a filtering portion and extending over the entire height of the filter (30), said side portion (34) extending at least opposite from the outlet for discharging filtered foodstuffs. According to the invention, the filtering side portion(s) (32a, 32b) of the filter (30) lead(s) to the top of the inclined portion(s) (42) of a bottom of the work chamber (40) opening toward the outlet for discharging filtered foodstuffs.

The invention relates to a split type vacuum electrostatic imprint extraction device and an application method thereof. The extraction device comprises a power supply module, a high voltage electrostatic generation module and a vacuumizing structure. The power supply module is electrically connected with the high voltage electrostatic generation module and the vacuumizing structure. The high voltage electrostatic generation module is used for converting low voltage direct current power into high voltage direct current power for output via high frequency oscillation, so as to generate continuous direct current high voltage static electricity. The vacuumizing structure includes a vacuum chamber and a vacuum pump connected with the vacuum chamber, and the vacuum pump is connected with a control module. The inventive split type vacuum electrostatic imprint extraction device has diversified functions, and is portable, wide in application range, practical and high in performance-to-price ratio.

The invention discloses separation and purification of gallic acid from suaeda salsa by SF-SMB, and relates to a method for separating and purifying the gallic acid from the suaeda salsa. In the wholeprocesses of extracting, separating and purifying the gallic acid from the suaeda salsa, water or ethanol which is environmentally-friendly to equipment and environments is taken as a solvent, and the solvent is conveniently recovered and the energy consumption is low; flavonoids constituents are not stable and easily oxidized, especially more susceptibly oxidized in high temperature environments, the separation and purification of the gallic acid from the suaeda salsa can be completed at room temperature by using an extraction method, the highest extraction temperature is controlled to be not higher than 70 DEG C, and conditions are relatively mild, thereby not affecting the extraction effect of flavonoids constituents, and further being beneficial to the stable existence of the flavonoids constituents; and by adopting the method of the invention, the continuity is enhanced, the purity of the gallic acid is high, the production cycle is shortened, and the cost is low.

The invention discloses a sectional steel pulling-out method for a SMW construction method pile. The method comprises the following steps: wrapping a layer of PVC sleeve pipe with a shape which is thesame as the shape of the outer surface of I-shaped sectional steel for stirring construction in the length direction of the outer surface of the I-shaped sectional steel; after the stirring construction is concluded, locating a crane in place, then connecting a hook crane with a hoisting hole of the I-shaped sectional steel and pulling out the I-shaped sectional steel, starting to grout through reserved grouting pipes, and realizing the synchronization of the pulling-out for the I-shaped sectional steel and the grouting. According to the method, the PVC sleeve with the shape which is the sameas the shape of the I-shaped sectional steel is adopted for wrapping the I-shaped sectional steel and inserted into a stirring pile with the I-shaped sectional steel, so that the instability of pulling resistance for the I-shaped sectional steel is avoided; with the adoption of the synchronization of the pulling-out for the I-shaped sectional steel and the grouting, a pressure on the bottom surface of the PVC sleeve, of the grouting and an upward pulling force on the I-shaped sectional steel, of the crane, friction resistance between the PVC sleeve and the I-shaped sectional steel is overcome, and the I-shaped sectional steel can be smoothly pulled out; and moreover, due to the existence of the PVC sleeve, the section of a space after the sectional steel is pulled out can be stabilized. The method is applicable to other sectional steel of the SMW construction method pile.

The invention relates to the technical field of DNA extraction, and provides a cell lysis buffer, a kit and a method for yeast genome extraction. The cell lysis buffer for extracting the yeast genome is prepared from the following components: 50 to 60 g/L of guanidine isothiocyanate, 1 to 10 g/L of Tris-base, 8 to 15 g/L of EDTA.2Na and 1 to 10 percent of Tween-20. Compared with the prior art, the yeast genome extraction kit provided by the invention is time-saving, labor-saving and thorough in cell lysis, chloroform is not used in the experiment process, the harm to experiment operators is reduced, and the extracted genome is high in yield and can be directly used for subsequent experiments, and does not need to be purified.

The invention provides a multiple RT-PCR detection kit for American type porcine reproductive and respiratory syndrome virus and porcine parvovirus, comprising a primer pair 1 for amplifying a target gene of American type porcine reproductive and respiratory syndrome virus and a primer pair 2 for amplifying a target gene of porcine parvovirus. The invention also provides a use method of the detection kit. The detection kit provided by the invention is quick as compared with the classical method of virus isolation, single RT-PCR and serotype typing, and can conveniently identify and distinguish any one of the American type porcine reproductive and respiratory syndrome virus and porcine parvovirus once, so that timely and quick response can be made to epidemic disease conditions, regions and environments, and a correct method of disposition can be decided in the shortest time, which has a positive meaning for stopping or blocking infectious disease transmission; and simultaneously the detection kit is especially suitable for extraction of small samples and has broad market application prospects.

The invention relates to a method for extracting plasmids with endotoxin removed on a large scale. The method comprises the steps of acquiring roughly-separated plasmid DNA by virtue of a classical alkaline lysis method, sequentially adding isopropanol, an NH4Ac solution, RNase A and NaCl, adding a mixed solution of PEG6000 and NaCl, dissolving and precipitating by virtue of a TE solution, repeatedly precipitating by virtue of absolute ethyl alcohol, washing and precipitating by virtue of 70% alcohol, simultaneously coordinating with centrifugation, drying, and finally dissolving and precipitating by virtue of the TE solution, so as to obtain target plasmids. According to the method, the pollution caused by endotoxin to plasmid DNA can be well removed, all reagents are standing chemical reagents in a laboratory, and the operation method is simple, easy to control and suitable for large-scale extraction of plasmids. The concentration of the plasmids can reach 5mg/mL, the endotoxin is lower than 5EU/mu g, and the plasmids can be applied to digestion, DNA sequencing, cell transfection, virus packaging and clinic animal immunization experiments.

The invention discloses a novel target tracking and positioning method based on pixel characteristics, which comprises the following steps of: removing human body region: extracting foreground by three frame difference methods for RGB components of pixel points of an image, and binarizing the foreground image to obtain a binary image; detecting the key points of human body in binary image, including hand key points, eyebrow center key points and bipedal midpoint; Marking all connected regions of the binary image; Target tracking and positioning: according to the pixel distribution characteristics of the connected region, the target position and the target positioning point are determined. Motion feature correction: According to the pixel distribution characteristics in the detected connected region, the vector direction of the region is obtained by dimension reduction method and the position of the target point is corrected. The method of the invention can quickly extract motion features, and can quickly and accurately track and locate a moving target in either a simple background or a complex scene, especially when the moving time is short and the target is long and slender, the method is very practical, and has a good popularization prospect.

The invention relates to the technical field of grease extraction processes, in particular to a method for extracting acer truncatum oil by combining ultrahigh pressure with a subcritical extraction technology. The method comprises the following steps: selecting raw materials; shelling: selecting acer truncatum seeds; crushing; ultrahigh-pressure treatment: performing ultrahigh-pressure treatmenton the acer truncatum seed powder under certain conditions; subcritical extraction: weighing about 100-300g of powder, putting the powder into a material bag, putting the material bag into a subcritical extraction tank, and vacuumizing the extraction tank until the vacuum degree is -0.1 Mpa; taking butane as an extracting agent, and extracting for 3-5 times under the conditions that the extractiontemperature is 20-50 DEG C, the extraction time is 30-120 minutes; and separation: after the extraction is finished, introducing the extraction liquid into a separation tank, reducing the pressure to-0.1 Mpa, and separating butane from the acer truncatum oil to obtain the acer truncatum oil. The extraction time is short; the energy consumption is low; and the oil yield is high.

The invention relates to a method for separating and purifying quercetin in common seepweed herb, in particular to a method for separating and purifying quercetin in common seepweed herb by use of a supercritical simulated moving bed chromatographic system. The whole process of extracting, separating and purifying quercetin from common seepweed herb adopts water or ethyl alcohol which is friendlyto equipment and the environment as a solvent, the solvent is convenient to recover, and the energy consumption is low. As flavonoid constituents are unstable, have high probability of being oxidized,and have probability of being oxidized particularly in a high-temperature environment. The adopted extraction method can be finished at the room temperature; the extraction temperature is controllednot to exceed 70 DEG C; and the condition is mild; not only is the flavonoid constituent extraction effect not influenced, but also stable existence of the flavonoid constituents is facilitated. By adoption of the method, the continuity is enhanced, the purity of gallic acid is high, the production cycle is shortened, and the cost is low.

The invention relates to a plasmid purification method for industrial production, which comprises the following steps: (1) splitting a bacterial culture containing plasmids to be purified to obtain a lysate; (2) adding a calcium chloride solution with the final concentration of 0.5-1M into the lysate to obtain a crude extract; (3) filtering the crude extract by using a membrane filter to obtain filtrate; and (4) carrying out chromatography on the filtrate by using an anion exchange column to obtain purified plasmids.