Method for extracting rice bran protein by complex enzyme

The technology of rice bran protein and compound enzyme is applied in the field of extracting vegetable protein, which can solve problems such as product bitterness, and achieve the effects of low production cost, good quality and simple process.

Inactive Publication Date: 2007-08-08

NORTHEAST AGRICULTURAL UNIVERSITY

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AI-Extracted Technical Summary

Problems solved by technology

Enzyme treatment is an effective and safe new method for extracting rice bran protein. In the previous research, the inventor of the present application used cellulase, compound protease, and phytase to extract rice bran protein isolate, so that the extraction rate of protein can be improved. , but the hig...

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Abstract

The invention discloses a purifying method of rice bran protein through composite enzyme, which comprises the following steps: allocating 10%-20% slurry with degreased rice bran and water; adjusting pH value to 5.0-6.5 and temperature to 40-50 deg.c; building up phytase, hemicellulase, cellulase and aspergillus protein oryzae with rate at 15.64í 15.06í60.78í8.52; adding 2-4% composite enzyme; stirring 1.5-2h; centrifuging 20 min; obtaining the supernatant as rice bran protein solution; condensing in the vacuum; freezing; drying; obtaining the product with protein receiving rate to 90.5-92.7%.

Application Domain

Fermentation

Technology Topic

ChemistryPhytase +6

Examples

Experimental program(5)

Example Embodiment

[0034] Example 1

[0035] 1. Weigh 100 g of defatted rice bran, add 900 mL of water, stir and disperse, prepare a slurry with a weight ratio of 10%, adjust the pH to 5.0-5.5 with HCl solution, and heat to 40±1°C.

[0036] 2. Add 0.31g of phytase, 0.3g of hemicellulase, 1.21g of cellulase, and 0.17g of aspergillus protease, mix well, enzymolyze in a 40±1℃ water bath, stir for 1.5 hours and adjust pH The value is maintained at 5.0 to 5.5.

[0037] 3. After 1.5 hours of enzymatic hydrolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r/min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0038] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure, and 12.2 g of rice bran protein was recovered by freeze drying. The degree of protein hydrolysis was 3.08%, and the extraction rate was 87.14%.

Example Embodiment

[0039] Example 2

[0040] 1. Weigh 150g of defatted rice bran, add 850mL of water, stir and disperse to prepare a slurry with a weight ratio of 15%, adjust the pH to 5.5-6.0 with HCl solution, and heat to 42±1°C.

[0041] 2. Add 0.47g of phytase, 0.45g of hemicellulase, 1.82g of cellulase, 0.26g of aspergillus protease, mix well, enzymolyze in a 42±1℃ water bath, stir for 1.5 hours, adjust pH The value is maintained at 5.5 to 6.0.

[0042] 3. After 1.5 hours of enzymatic hydrolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r/min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0043] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure and freeze-dried to recover 15.5 g of rice bran protein, with a protein hydrolysis degree of 3.76% and an extraction rate of 73.81%.

Example Embodiment

[0044] Example 3

[0045] 1. Weigh 200 g of defatted rice bran, add 800 mL of water, stir and disperse to prepare a slurry with a weight ratio of 20%, adjust the pH to 5.0-5.5 with HCl solution, and heat to 45±1°C.

[0046]2. Add 1.25g of phytase, 1.20g of hemicellulase, 4.86g of cellulase, and 0.68g of aspergillus protease, mix well, enzymolyze in a water bath at 45±1℃, stir for 2 hours, adjust pH The value is maintained at 5.0 to 5.5.

[0047] 3. After 2 hours of enzymolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r/min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0048] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure and freeze-dried to recover 23.78 g of rice bran protein, with a protein hydrolysis degree of 4.06% and an extraction rate of 84.93%.

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Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

Good quality
Stable performance

Method for extracting rice bran protein by complex enzyme

The technology of rice bran protein and compound enzyme is applied in the field of extracting vegetable protein, which can solve problems such as product bitterness, and achieve the effects of low production cost, good quality and simple process.

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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