Method for extracting rice bran protein by complex enzyme

The technology of rice bran protein and compound enzyme is applied in the field of extracting vegetable protein, which can solve problems such as product bitterness, and achieve the effects of low production cost, good quality and simple process.

Inactive Publication Date: 2007-08-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme treatment is an effective and safe new method for extracting rice bran protein. In the previous research, the inventor of the present application used cellulase, compound protease, and phytase to extract rice bran protein isolate, so that the extraction rate of protein can be improved. , but the hig

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] 1. Weigh 100 g of defatted rice bran, add 900 mL of water, stir and disperse, prepare a slurry with a weight ratio of 10%, adjust the pH to 5.0-5.5 with HCl solution, and heat to 40±1°C.

[0036] 2. Add 0.31g of phytase, 0.3g of hemicellulase, 1.21g of cellulase, and 0.17g of aspergillus protease, mix well, enzymolyze in a 40±1℃ water bath, stir for 1.5 hours and adjust pH The value is maintained at 5.0 to 5.5.

[0037] 3. After 1.5 hours of enzymatic hydrolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r / min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0038] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure, and 12.2 g of rice bran protein was recovered by freeze drying. The degree o...

Example Embodiment

[0039] Example 2

[0040] 1. Weigh 150g of defatted rice bran, add 850mL of water, stir and disperse to prepare a slurry with a weight ratio of 15%, adjust the pH to 5.5-6.0 with HCl solution, and heat to 42±1°C.

[0041] 2. Add 0.47g of phytase, 0.45g of hemicellulase, 1.82g of cellulase, 0.26g of aspergillus protease, mix well, enzymolyze in a 42±1℃ water bath, stir for 1.5 hours, adjust pH The value is maintained at 5.5 to 6.0.

[0042] 3. After 1.5 hours of enzymatic hydrolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r / min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0043] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure and freeze-dried to recover 15.5 g of rice bran protein, with a protein hydrolysis ...

Example Embodiment

[0044] Example 3

[0045] 1. Weigh 200 g of defatted rice bran, add 800 mL of water, stir and disperse to prepare a slurry with a weight ratio of 20%, adjust the pH to 5.0-5.5 with HCl solution, and heat to 45±1°C.

[0046]2. Add 1.25g of phytase, 1.20g of hemicellulase, 4.86g of cellulase, and 0.68g of aspergillus protease, mix well, enzymolyze in a water bath at 45±1℃, stir for 2 hours, adjust pH The value is maintained at 5.0 to 5.5.

[0047] 3. After 2 hours of enzymolysis, terminate the reaction, centrifuge in a high-speed centrifuge for 20 minutes, centrifuge at 3000r / min, and collect the centrifuged liquid. The precipitate was first washed with 100 mL of water, and then with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifugal liquid was combined.

[0048] 4. The centrifugal liquid was concentrated by evaporation under reduced pressure and freeze-dried to recover 23.78 g of rice bran protein, with a protein hydrolysis degr...

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PUM

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Abstract

The invention discloses a purifying method of rice bran protein through composite enzyme, which comprises the following steps: allocating 10%-20% slurry with degreased rice bran and water; adjusting pH value to 5.0-6.5 and temperature to 40-50 deg.c; building up phytase, hemicellulase, cellulase and aspergillus protein oryzae with rate at 15.64í† 15.06í†60.78í†8.52; adding 2-4% composite enzyme; stirring 1.5-2h; centrifuging 20 min; obtaining the supernatant as rice bran protein solution; condensing in the vacuum; freezing; drying; obtaining the product with protein receiving rate to 90.5-92.7%.

Description

1. Technical field [0001] The invention relates to a method for extracting vegetable protein, in particular to a method for extracting protein from rice bran. 2. Background technology [0002] The annual output of rice in my country is 185 million tons, and the output of rice bran is about 10 million tons. Rice bran contains 12%-15% protein, which is higher than the content of 5%-7% in rice. At the same time, there are 37% albumin in rice bran protein. 36% globulin, 22% glutenin and 5% gliadin (Betschart, et al, 1977), the lysine content is higher than that in rice and other gluten (Juliano1985), which compensates for the lysine in the cereal protein The defect of acid deficiency greatly improves the nutritional value and is a high-quality protein raw material. Therefore, extracting rice bran protein has excellent economy and practicability. [0003] At present, there is no domestic manufacturer that can produce rice bran protein isolate. The most commonly used method for e...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 徐红华王雪飞
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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