Value Addition of Engine-Usable Fatty Acid Methyl Esters (Biodiesel) and By-Products from Naturally Occurring Marine Microalgae Populations and Marine Microalgae Cultivated in Open-air Salt Pans

一种脂肪酸甲酯、生物柴油的技术，应用在脂肪酸酯化、脂油/脂肪生产、生物燃料等方向，能够解决耗能等问题

Inactive Publication Date: 2016-06-29
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods of producing biodiesel from algae are very energy-intensive

Method used

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  • Value Addition of Engine-Usable Fatty Acid Methyl Esters (Biodiesel) and By-Products from Naturally Occurring Marine Microalgae Populations and Marine Microalgae Cultivated in Open-air Salt Pans
  • Value Addition of Engine-Usable Fatty Acid Methyl Esters (Biodiesel) and By-Products from Naturally Occurring Marine Microalgae Populations and Marine Microalgae Cultivated in Open-air Salt Pans
  • Value Addition of Engine-Usable Fatty Acid Methyl Esters (Biodiesel) and By-Products from Naturally Occurring Marine Microalgae Populations and Marine Microalgae Cultivated in Open-air Salt Pans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] With the help of GoogleMaps TM , which looks for green spots in coastal waters, which can help us identify possible populations of floating microalgae. Some distinct green spots were found in the coastal areas of Goa (Madkai; 15°41.0616'N, 73°95.6227'E), Kerala (Vellanathuruthu Road; 9°01.6659'N, 76°52.5022'E), Bengal (PortCanning, 22°31.5577'N, 88°67.3307'E;, Dongajora, 22°13.2696'N, 88°60.2676'E; Haldiarefinery, 22°04.9408'N, 88°07.308'E), Diu (Nagoa Road Beside, 70□54.959'E, 20□42.391N) and Gujarat (Okha, 68□59.876', 22□23.975'). Ground-truth measurements were carried out at the sites identified in Diu and Gujarat, and indeed floating populations of green microalgae were found.

Embodiment 2

[0116] The algal populations of Example 1 were collected and observed under a microscope (Carl Zeiss AxioImager, 40x) for taxonomic identification. Both populations showed consortia of microalgae in which the Chlorophycae family predominated. The algae collected from 70□54.959'E, 20□42.391N had Microsporidium as the dominant form, while the algae collected from 68□59.876', 22□23.975' had Cladoides as the dominant form. Isolation of related species of the consortium was performed by using serial dilution methods. The seaweed algae were washed with distilled water to remove adhering dirt and impurities, and further centrifuged. Supernatants were collected and seeded in 24-well tissue culture plates with different media (BG-11, BBM, Zarrouk's, ASN-III, etc.). Serial dilutions were performed using a 1:10 dilution. Tissue culture plates were maintained at 25°C under artificial light (300 lux) with a 12 hour light and dark cycle. After visible growth, the enriched culture was st...

Embodiment 3

[0118] Naturally occurring populations of marine microalgae were selected from microalgae predominating at the site longitude 70°54.959'E and latitude 20°42.391N. The site was inspected regularly after 3-4 weeks to study regrowth of algal blooms. During the summer, the biomass production rate was 22.22g / m 2 / day and a total lipid content of 10%; during the monsoon, the biomass productivity was 6.03g / m 2 / day and a total lipid content of 9.61%, while in winter, 16g / m 2 / day biomass productivity and 12.85% total lipid content. This example teaches us that it is possible to harvest microalgal populations from nature in a sustainable manner.

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Abstract

The present invention teaches the obtained instructions and method for producing engine-useable marine microalgal fatty acid methyl esters (biodiesel) using naturally occurring populations of marine microalgae and culturing marine microalgae in sun-dried salt pans in a cost-effective manner . The utility of by-product streams adds to the attractiveness of the present invention.

Description

field of invention [0001] The present invention relates to the use of naturally occurring lipid-containing microalgae collected from the west coast of India for the production of fatty acid methyl esters (FAME) and demonstrates the suitability of the latter for common vehicle travel. The present invention also relates to the cultivation of readily harvestable lipid-containing microalgal biomass (cultured from Chlorella variabilis accession number PTA-12198) in sun-dried salt pans and its further conversion to FAMEs that can also be used in engines . Background of the invention [0002] Can refer to the article of Daemon Fairless, Biofuel: The little shrub that could–maybe (biodiesel: small shrub can-can be). Life Cycle Assessment), Environmental Science & Technology (2009) 43:17, 6475-6481, which highlights the complex issue of food versus fuel and emphasizes the need for such biomass sources that do not affect food production. [0003] See also D.H. Lee, Algal biodiesel e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C10L1/02
CPCC10L1/026C10G2300/1014C10G2300/302C10G2300/308C10G2300/44C11B1/10C11B3/00C11C3/06C11C3/10Y02P30/20C12P7/649Y02E50/10Y02P20/133C10L1/02C12P7/64C11C3/04
Inventor 桑迪亚·钱德里卡·普拉萨德·米什拉普什皮托·库马·高希马赫什·拉姆尼可拉尔·甘地苏里希·巴塔查里亚苏巴尔纳·迈蒂苏梅希·钱德拉·乌帕德亚阿吕普·高希拉沙普迪·巴德里·纳拉亚纳·普拉萨德桑吉特·卡尼维尔桑吉夫·库马·米什拉阿努帕玛·维贾伊库马·什里瓦斯塔伊姆兰·班差凯坦·帕利瓦托莫伊·高希拉胡尔·库马·毛里亚蒂普提·贾殷沙里希·库马·帕蒂达阿比谢克·萨胡赫塔·波萨米亚克鲁希纳德辛·萨拉
Owner COUNCIL OF SCI & IND RES
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