Cell lysis buffer, kit and method for yeast genome extraction

Abstract

The invention relates to the technical field of DNA extraction, and provides a cell lysis buffer, a kit and a method for yeast genome extraction. The cell lysis buffer for extracting the yeast genome is prepared from the following components: 50 to 60 g/L of guanidine isothiocyanate, 1 to 10 g/L of Tris-base, 8 to 15 g/L of EDTA.2Na and 1 to 10 percent of Tween-20. Compared with the prior art, the yeast genome extraction kit provided by the invention is time-saving, labor-saving and thorough in cell lysis, chloroform is not used in the experiment process, the harm to experiment operators is reduced, and the extracted genome is high in yield and can be directly used for subsequent experiments, and does not need to be purified.

Description

technical field [0001] The invention relates to the technical field of DNA extraction, in particular to a cell lysate for yeast genome extraction, a kit containing the lysate and a method for extracting yeast genome using the kit. Background technique [0002] Yeast is often used as a model system for eukaryotic genome expression because of its clear genetic background, easy operation, and rapid growth. In addition, yeast is widely used in genetic engineering and food production. In the process of studying yeast, extracting its genome for detection of target genes is an important step. [0003] The yeast cell wall is tough and thick, with a thickness of 0.1-0.3 μm, and its weight accounts for 18%-30% of the dry weight of the cell. It plays an important role in maintaining cell shape and intercellular recognition. Its structure is similar to a sandwich. The outer layer is mannan, the middle layer is protein molecules, and the inner layer is glucan. The main chain of manna...

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction of yeast genome is costly, time-consuming, and of poor quality. There

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