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Cell lysis buffer, kit and method for yeast genome extraction

A cell lysate and genome technology, applied in recombinant DNA technology, DNA preparation, etc., can solve the problems of long time, poor quality, and high cost of yeast genome extraction, and achieve high success rate, better effect, and shorten the lysis time. Effect

Pending Publication Date: 2021-08-17
JIERUI BIOENG SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the extraction of yeast genome is costly, time-consuming, and of poor quality. Therefore, an improved and optimized yeast genome extraction method and related products are urgently needed

Method used

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  • Cell lysis buffer, kit and method for yeast genome extraction
  • Cell lysis buffer, kit and method for yeast genome extraction

Examples

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Embodiment 1

[0039] This embodiment provides a cell lysate for yeast genome extraction, including components and their contents as follows: guanidine isothiocyanate 54.2g / L, Tris-base 3.6g / L, EDTA 2Na 9.4g / L L. Tween-202%.

Embodiment 2

[0041] This example provides a cell lysate for yeast genome extraction, including components and their contents as follows: guanidine isothiocyanate 60g / L, Tris-base 2.4g / L, EDTA 2Na 12g / L, Tween - 205%.

Embodiment 3

[0043] This example provides a cell lysate for yeast genome extraction, including components and their contents as follows: guanidine isothiocyanate 50g / L, Tris-base 4.2g / L, EDTA·2Na 5.4g / L , Tween -201%.

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Abstract

The invention relates to the technical field of DNA extraction, and provides a cell lysis buffer, a kit and a method for yeast genome extraction. The cell lysis buffer for extracting the yeast genome is prepared from the following components: 50 to 60 g / L of guanidine isothiocyanate, 1 to 10 g / L of Tris-base, 8 to 15 g / L of EDTA.2Na and 1 to 10 percent of Tween-20. Compared with the prior art, the yeast genome extraction kit provided by the invention is time-saving, labor-saving and thorough in cell lysis, chloroform is not used in the experiment process, the harm to experiment operators is reduced, and the extracted genome is high in yield and can be directly used for subsequent experiments, and does not need to be purified.

Description

technical field [0001] The invention relates to the technical field of DNA extraction, in particular to a cell lysate for yeast genome extraction, a kit containing the lysate and a method for extracting yeast genome using the kit. Background technique [0002] Yeast is often used as a model system for eukaryotic genome expression because of its clear genetic background, easy operation, and rapid growth. In addition, yeast is widely used in genetic engineering and food production. In the process of studying yeast, extracting its genome for detection of target genes is an important step. [0003] The yeast cell wall is tough and thick, with a thickness of 0.1-0.3 μm, and its weight accounts for 18%-30% of the dry weight of the cell. It plays an important role in maintaining cell shape and intercellular recognition. Its structure is similar to a sandwich. The outer layer is mannan, the middle layer is protein molecules, and the inner layer is glucan. The main chain of manna...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/101
Inventor 龙桂梅楚思远陈茂阳肖爱军
Owner JIERUI BIOENG SHANGHAI
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