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Special efficient culture medium for necrotic fusobacterium and preparation method thereof

A Fusobacterium necroptosis, culture medium technology, applied in the high-efficiency medium for Fusobacterium necroptosis and its preparation field, can solve the problems of no growth, high reduction potential, low nutrient oxidation, etc., and achieve vigorous bacterial growth, large bacterial output, and low price cheap effect

Inactive Publication Date: 2009-09-02
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Fusobacterium necroptosis is a type of obligate anaerobic bacteria that can only grow under low oxygen partial pressure conditions. The bacteria lack a complete metabolic enzyme system, and energy metabolism is carried out by anaerobic fermentation, and anaerobic fermentation obtains energy more than aerobic oxidation The energy produced is much lower. The existing Fusobacterium necrotica medium is generally mixed with beef broth, blister medium and liver brain juice. Due to the low oxidation of nutrients and high reduction potential, the growth of bacteria is not vigorous or does not grow, and cannot To meet the needs of vaccine production or research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Collect and process the main raw materials of the medium, including beef, porcine pancreas and liver; process the beef, porcine pancreas and liver: remove fascia and fat, cut the liver and some beef into small pieces, and some beef and porcine pancreas mince for later use;

[0015] 2. Preparation of basal culture medium 1: Put 18Kg of minced beef and 30Kg of distilled water into a container, heat to 40 0 C, adjust Ph8.2 with 20% NaOH, add 4Kg of minced pig pancreas and 800mL of chloroform, 42 0 C, place it for 3 days, adjust the pH value to 5.4 with concentrated hydrochloric acid, boil and filter, discard the meat residue for later use;

[0016] Prepare basal culture medium 2: Slice 500g of pig liver, add 2500g of distilled water, boil for 30min, filter and discard the meat dregs for later use;

[0017] Prepare basal culture medium 3: slice 500g of beef, add 2500g of distilled water, boil for 30min, filter and discard the meat residue for later use;

[0018] 3. Ta...

Embodiment 2

[0020] 1. Collect and process the main raw materials of the medium, including beef, porcine pancreas and liver; process the beef, porcine pancreas and liver: remove fascia and fat, cut the liver and some beef into small pieces, and some beef and porcine pancreas mince for later use;

[0021] 2. Preparation of basal culture medium 1: Put 18Kg of minced beef and 30Kg of distilled water into a container, heat to 40 0 C, adjust Ph8.2 with 20% NaOH, add 4Kg of minced pig pancreas and 800mL of chloroform, 42 0 C, place it for 3 days, adjust the pH value to 5.4 with concentrated hydrochloric acid, boil and filter, discard the meat residue for later use;

[0022] Prepare basal culture medium 2: Slice 500g of pig liver, add 2500g of distilled water, boil for 30min, filter and discard the meat dregs for later use;

[0023] Prepare basal culture medium 3: slice 500g of beef, add 2500g of distilled water, boil for 30min, filter and discard the meat residue for later use;

[0024] 3. Ta...

Embodiment 3

[0026] 1. Collect and process the main raw materials of the medium, including beef, porcine pancreas and liver; process the beef, porcine pancreas and liver: remove fascia and fat, cut the liver and some beef into small pieces, and some beef and porcine pancreas mince for later use;

[0027] 2. Preparation of basal culture medium 1: Put 18Kg of minced beef and 30Kg of distilled water into a container, heat to 40 0 C, adjust Ph8.2 with 20% NaOH, add 4Kg of minced pig pancreas and 800mL of chloroform, 42 0 C, place it for 3 days, adjust the pH value to 5.4 with concentrated hydrochloric acid, boil and filter, discard the meat residue for later use;

[0028] Prepare basal culture medium 2: Slice 500g of pig liver, add 2500g of distilled water, boil for 30min, filter and discard the meat dregs for later use;

[0029] Prepare basal culture medium 3: slice 500g of beef, add 2500g of distilled water, boil for 30min, filter and discard the meat residue for later use;

[0030] 3. Ta...

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Abstract

The invention relates to a special efficient culture medium for necrotic fusobacterium and a preparation method thereof. The culture medium mainly comprises carbon source substances and nitrogen source substances. The invention is characterized in that the culture medium is prepared from 500 to 1000ml of basal culture solution 1, 40 to 50g of stomach peptone, 500 to 1000ml of basal culture solution 2, 500 to 1000ml of basal culture solution 3, 15 to 18g of Na2HPO4H.H2O, 30g of NaCl, 5 to 10g of protohemin, 0.5 to 1g of vitamin K, 2.5 to 5g of cysteine hydrochloride and 100 to 200g of glucose by regulating the pH value to 7.4-7.8. When the culture medium is used for cultivating necrotic fusobacterium, the necrotic fusobacterium grows quickly, the necrotic fusobacterium yield is large, and the cost is low. The special efficient culture medium for necrotic fusobacterium is suitable for vaccine production or extraction of leukotoxin.

Description

Technical field: [0001] The invention relates to a special high-efficiency culture medium for fusobacterium necrosis and a preparation method thereof. Background technique: [0002] Fusobacterium necroptosis is a type of obligate anaerobic bacteria that can only grow under low oxygen partial pressure conditions. The bacteria lack a complete metabolic enzyme system, and energy metabolism is carried out by anaerobic fermentation, and anaerobic fermentation obtains energy more than aerobic oxidation The energy produced is much lower. The existing Fusobacterium necrotica medium is generally mixed with beef broth, blister medium and liver brain juice. Due to the low oxidation of nutrients and high reduction potential, the growth of bacteria is not vigorous or does not grow, and cannot To meet the needs of vaccine production or research. Invention content: [0003] The object of the present invention is to provide a special high-efficiency medium for Fusobacterium necroptosis a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/145
Inventor 陈立志王克坚杨福合刘晓颖程世鹏
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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