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Method for extracting rice bran protein by complex enzyme

A technology of rice bran protein and compound enzyme is applied in the field of extracting vegetable protein, which can solve problems such as product bitterness, and achieve the effects of low production cost, stable performance and good quality.

Inactive Publication Date: 2011-03-23
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme treatment is an effective and safe new method for extracting rice bran protein. In the previous research, the inventor of the present application used cellulase, compound protease, and phytase to extract rice bran protein isolate, so that the extraction rate of protein can be improved. , but the highest extraction rate using these enzymes can only reach 87%, and the extracted product has a certain bitter taste
Therefore, the main problem of enzymatic extraction of rice bran protein is to improve the protein yield under the premise of ensuring the function and nutritional properties of rice bran protein.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] 1. Weigh 100g of defatted rice bran, add 900mL of water, stir and disperse, and prepare a slurry with a weight ratio of 10%, adjust the pH value to 5.0-5.5 with HCl solution, and heat to 40±1°C.

[0036] 2. Add 0.31g of phytase, 0.3g of hemicellulase, 1.21g of cellulase, and 0.17g of rice koji protease, mix well, enzymolyze in a water bath at 40±1°C, stir for 1.5 hours, and adjust the pH The value is maintained at 5.0-5.5.

[0037] 3. After 1.5 hours of enzymatic hydrolysis, stop the reaction, centrifuge in a high-speed centrifuge for 20 minutes at a speed of 3000r / min, and collect the centrifugate. The precipitate was first washed with 100 mL of water, and then washed with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifuged solution was combined.

[0038] 4. The centrifuge was evaporated and concentrated under reduced pressure, and 12.2 g of rice bran protein was recovered by freeze-drying, the degree of protein hyd...

example 2

[0040] 1. Weigh 150g of defatted rice bran, add 850mL of water, stir and disperse, and prepare a slurry with a weight ratio of 15%, adjust the pH value to 5.5-6.0 with HCl solution, and heat to 42±1°C.

[0041] 2. Add 0.47g of phytase, 0.45g of hemicellulase, 1.82g of cellulase, and 0.26g of rice koji protease, mix well, enzymolyze in a water bath at 42±1°C, stir for 1.5 hours, and adjust the pH The value is maintained at 5.5-6.0.

[0042] 3. After 1.5 hours of enzymatic hydrolysis, stop the reaction, centrifuge in a high-speed centrifuge for 20 minutes at a speed of 3000r / min, and collect the centrifugate. The precipitate was first washed with 100 mL of water, and then washed with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifuged solution was combined.

[0043] 4. The centrifuge was evaporated and concentrated under reduced pressure, and 15.5 g of rice bran protein was recovered through freeze-drying, the degree of prote...

example 3

[0045] 1. Weigh 200g of defatted rice bran, add 800mL of water, stir and disperse to prepare a slurry with a weight ratio of 20%, adjust the pH value to 5.0-5.5 with HCl solution, and heat to 45±1°C.

[0046]2. Add 1.25g of phytase, 1.20g of hemicellulase, 4.86g of cellulase, and 0.68g of rice koji protease, mix well, enzymolyze in a water bath at 45±1°C, stir for 2 hours, and adjust the pH The value is maintained at 5.0-5.5.

[0047] 3. After 2 hours of enzymatic hydrolysis, stop the reaction, centrifuge in a high-speed centrifuge for 20 minutes at a speed of 3000r / min, and collect the centrifugate. The precipitate was first washed with 100 mL of water, and then washed with 200 mL of ethanol solution, the ethanol concentration was 80%, and then centrifuged, and the centrifuged solution was combined.

[0048] 4. The centrifuge was evaporated and concentrated under reduced pressure, and 23.78 g of rice bran protein was recovered through freeze-drying, the degree of protein hyd...

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Abstract

The invention discloses a purifying method of rice bran protein through composite enzyme, which comprises the following steps: allocating 10%-20% slurry with degreased rice bran and water; adjusting pH value to 5.0-6.5 and temperature to 40-50 deg.c; building up phytase, hemicellulase, cellulase and aspergillus protein oryzae with rate at 15.64:15.06:60.78:8.52; adding 2-4% composite enzyme; stirring 1.5-2h; centrifuging 20 min; obtaining the supernatant as rice bran protein solution; condensing in the vacuum; freezing; drying; obtaining the product with protein receiving rate to 90.5-92.7%.

Description

1. Technical field [0001] The invention relates to a method for extracting vegetable protein, in particular to a method for extracting protein from rice bran. 2. Background technology [0002] The annual output of rice in my country is 185 million tons, and the output of rice bran is about 10 million tons. Rice bran contains 12%-15% protein, which is higher than the content of 5%-7% in rice. At the same time, there are 37% albumin in rice bran protein. 36% globulin, 22% glutenin and 5% gliadin (Betschart, et al, 1977), the lysine content is higher than that in rice and other gluten (Juliano1985), which compensates for the lysine in the cereal protein The defect of acid deficiency greatly improves the nutritional value and is a high-quality protein raw material. Therefore, extracting rice bran protein has excellent economy and practicability. [0003] At present, there is no domestic manufacturer that can produce rice bran protein isolate. The most commonly used method for e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 徐红华王雪飞李铁晶于国萍邵美丽张立钢
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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