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Cell preservation liquid, application thereof, and method for preserving cells

A preservation solution and cell technology, applied in the field of cell preservation solution, can solve the problems of low cell viability and death, achieve high survival rate, reduce adhesion, and reduce the effect of cell mass embolism

Inactive Publication Date: 2016-08-24
YIDA INT BIOTECH BEIJING CO LTD
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a cell preservation solution and its use and method for preserving cells, so as to solve the technical problems in the prior art such as low cell viability and mass death after long-term transportation of cells

Method used

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  • Cell preservation liquid, application thereof, and method for preserving cells
  • Cell preservation liquid, application thereof, and method for preserving cells
  • Cell preservation liquid, application thereof, and method for preserving cells

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Experimental Example 1 Preparation of cell preservation solution of the present invention

[0039] experiment procedure

[0040] 1. Preparation of 0.9% normal saline containing negative H ions: take 100ml 3% NaCl in a sterile environment, dilute to 0.9% with an appropriate amount of negative H ion water (made by the laboratory according to the device described in the manual), and constant volume.

[0041] 2. Carry out Gram detection, endotoxin detection, sterility and mycoplasma detection with the water containing negative H ions in constant volume, and confirm that there is no endotoxin, sterility and mycoplasma contamination.

experiment example 2

[0042] Selection of Negative Hydrogen Ion Concentration in Experimental Example 2 Cell Preservation Solution (600ppb H-, 400ppb H-, 200ppb H-)

[0043] 1. According to the method described in the above-mentioned experimental example 1, the cell preservation solutions with negative hydrogen ion concentrations of 600ppb H-, 400ppb H-, and 200ppb H- were prepared respectively.

[0044] 2. Preparation of MSC and CIK cells

[0045] MSCs and CIKs used in this experiment (mesenchymal stem cells prepared from umbilical cord tissue in this laboratory, and cytokine-induced killer T cells derived from umbilical cord blood). In addition, PBS herein has the usual meaning in this field, and the composition of PBS is preferably KCl0.2g / L in this experimental example; KH 2 PO 4 0.2g / L; NaCl8g / L; NaCl 2 HPO 4 1.15g / L; Ph7.2-7.4; osmotic pressure 275+15.

[0046] 1) Collect the cultured MSC and CIK cells, adjust the cell density to 1×10 5 / ml. Aspirate the culture solution, add 5ml of PB...

experiment example 3

[0063] Experimental Example 3 Effects of the aqueous cell solution of the present invention on the activity of umbilical cord mesenchymal stem cells and CIK cells at different concentrations.

[0064] Select the physiological saline with negative hydrogen ion concentration as 600ppb H- as the cell preservation solution used in this experiment, and select the physiological saline without negative hydrogen ion as contrast, analyze the influence of cell preservation solution of the present invention on different cell concentrations. The specific results are shown in Table 2 below.

[0065] Table 2. Effect of cell preservation solution on different cell concentrations.

[0066]

[0067] Table 2 (continued)

[0068]

[0069] Table 2 (continued)

[0070]

[0071] As shown in Table 2, the cell preservation solution showed excellent survival rates for different concentrations of CIK and MSC cells. However, when compared with physiological saline under the same conditions b...

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Abstract

The invention provides a cell preservation liquid, an application thereof, and a method for preserving cells. The cell preservation liquid is mainly prepared from a sodium chloride water solution being 0.5-1.5 g / ml in concentration and also includes a negative hydrogen ion substance which exists under a cell preservation condition. The cell preservation liquid can maintain high activity rate for a long period and reduce cell aggregation for cells, such as stem cells, during processes such as transportation.

Description

technical field [0001] The present invention relates to a technology for preserving cells, in particular to a cell preservation solution and its use and method for preserving cells. The technology can keep cells at a high activity rate for a long time and reduce cell aggregation during transportation and other processes. Background technique [0002] Various types of cells, especially stem cells, have extremely important uses in various fields such as life science research, drug screening tests, and tissue and organ repair research. For example, mesenchymal stem cells (MSC, Mesenchymal Stem Cell) have attracted increasing attention due to their characteristics of multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. [0003] However, there are still some problems in the application of these cells, especially stem cells, such as mesenchymal stem cells in scientific research, industry and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 王亚军杜静冯志磊吴瑜瑜田利明
Owner YIDA INT BIOTECH BEIJING CO LTD
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