Fluorogenic quantitative PCR detection reagent and preparation method and application thereof

A fluorescent quantitative and detection reagent technology, applied in the field of animal disease quarantine and detection, can solve problems such as economic losses in the poultry industry
CN105907893AActive Publication Date: 2016-08-31HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
Publication Date
2016-08-31

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Abstract

The invention discloses a fluorogenic quantitative PCR detection reagent for FAdV-4TaqMan MGB and a preparation method and application of the fluorogenic quantitative PCR detection reagent. The fluorogenic quantitative PCR detection reagent comprises a pair of specific primers and a fluorescence probe. The nucleotide sequences of the specific primers are SEQ ID NO.1-2, and the nucleotide sequence of the fluorescence probe is SEQ ID NO.3. The aviadenovirus I subgroup C-race forth serotype virus (FAdV-4)TaqMan MGB fluorogenic quantitative PCR detection primers and probe and an FQ-PCR detection method established based on the primers and probe have the advantages of being fast, specific, sensitive, high in flux and the like, detection can be completed within 1 h to 2 h, and the requirement for large-batch and fast detection of aviadenovirus I subgroup C-race forth serotype viruses can be met. An effective tool is provided for fast detection, infection source surveying, transmission environment and pathogeny tracing of aviadenovirus, and great significance is achieved for prevention and control of the viruses.
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Description

technical field

[0001] The invention belongs to the technical field of animal epidemic quarantine detection, and in particular relates to a TaqMan MGB fluorescent quantitative PCR detection reagent for avian adenovirus serotype 4 (FAdV-4) and a preparation method and application thereof. Background technique

[0002] Avian adenoviruses are divided into subgroup I, subgroup II, and subgroup III adenoviruses. Subgroup Ⅰ (Fowl adenovirus group Ⅰ, FAdVI) is a virus isolated from the respiratory tract of chickens, turkeys, geese and quails. The viruses of this subgroup are divided into 5 species, A, B, C, D, and E, with a total of 12 serotypes , There can be no cross-protection between viruses of each serotype. In the past many years, FAdVI mostly replicated in the body without becoming ill. After the animal was infected with the virus, it was a recessive infection, and it co-acted on poultry with other pathogens. However, since 2015, poultry with serotype 4 (FAdV-4) in subgrou...

Claims

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