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PCR primer pair for identification or auxiliary identification of black pork, identification method and application thereof

A technology of auxiliary identification and primer pairing is applied in the field of identification and detection of black pork to achieve the effect of standardizing the market, protecting rights and interests, and promoting fair competition

Inactive Publication Date: 2016-09-14
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no record of using the black hair gene to identify black pork

Method used

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  • PCR primer pair for identification or auxiliary identification of black pork, identification method and application thereof
  • PCR primer pair for identification or auxiliary identification of black pork, identification method and application thereof
  • PCR primer pair for identification or auxiliary identification of black pork, identification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Design and synthesis of PCR primers for identification or auxiliary identification of black pork

[0029] F:5'-AGCCATGAGCTGAGCAGGAC-3'

[0030] R: 5'-GAAGCAGAGGCTGGACACCAT-3'

[0031] Coat color is one of the important characteristics of animal breeds, and the coat color varies greatly among different pig breeds. The coat color trait can be used as a genetic marker, which can help to obtain the desired coat color type that can be stably inherited in the process of cross-breeding. The MC1R gene is a key gene in the regulation of animal melanin formation and plays an important role in determining the fur color of animals. MC1R controls the formation of melanin by combining with α-melanostimulating hormone (α-MSH) and adrenal cortex hormone (ACTH). It is activated by melanocyte-stimulating hormone (MSH), leading to increased formation of eumelanin (black and brown) in melanosomes, which are then transported via melanosomes into surrounding hair. Therefore, t...

Embodiment 2

[0033] Example 2. Kits for identification or auxiliary identification of black pork

[0034] After packaging the primers F and R designed and synthesized in Example 1 separately, package them in the same kit with separately packaged dNTP, DNA polymerase, 10×buffer buffer, etc., or package the primers F and R separately , packaged in the same kit with the SuperMix, that is, the kit for identifying or assisting in identifying black pork of the present invention is obtained.

Embodiment 3

[0035] Embodiment 3. Identify whether the meat sample to be tested is black pork

[0036] (1) Sample preparation

[0037] Select 3 purebred Laiwu black pigs, Da Pulian black pigs, Large White pigs, Duroc pigs, and hybrid Lulai black pigs, and Du Dachang Sanyuan commercial pigs, and extract genomic DNA from their muscle tissues. The extraction method for the general method.

[0038] (2) PCR amplification

[0039] Three copies of each of the genomic DNAs of the above-mentioned 6 pig breeds of Laiwu black pig, Dapulian black pig, Large White pig, Duroc pig, Lulai black pig, and Du Dachang Sanyuan commercial pig were carried out one by one with the primer pairs of the present invention. PCR amplification, and then gel electrophoresis to detect whether there are corresponding amplified bands.

[0040] The total PCR amplification reaction system is 25 μL, including 12.5 μL of SuperMix, 0.8 μL of upstream and downstream primers (10 pmol / μl), 1 μL of template (100 ng / μl), ddH 2 O ...

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PUM

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Abstract

The invention provides a PCR primer pair for identification or auxiliary identification of black pork, an identification method and application thereof. Sequence of the PCR primer pair for identification or auxiliary identification of black pork is F:5'-AGCCATGAGCTGAGCAGGAC-3'; R:5'-GAAGCAGAGGCTGGACACCAT-3'. The primer pair is used for PCR amplification of DNA of pork to be tested. According to sequencing results of amplified bands, whether the pork to be tested is black pork can be judged. According to the invention, provenance of pork can be determined accurately, and the identification result is accurate and efficient.

Description

technical field [0001] The invention relates to a PCR primer pair, an identification method and an application for identifying or assisting identification of black pork, and relates to the technical field of molecular markers of black pork, in particular to the identification and detection technology of black pork. Background technique [0002] Meat production occupies an extremely important position in human life. Since 1990, the total output and consumption of meat products in my country have always ranked first in the world. From the perspective of consumption structure, the pork consumption of Chinese residents accounts for all meat in the country. More than 60% of the total consumption. With the development of society and the gradual improvement of residents' consumption level, the traditional supply and marketing model is difficult to meet people's growing needs for material civilization. More and more consumers are beginning to pay attention to food safety, health and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q1/6869C12Q2531/113
Inventor 蔺海朝王彦平王继英郭建凤成建国林松
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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