A method of cultivating clean-level experimental tree shrews by drug purification and screening

Abstract

The invention relates to a method for breeding clean experimental tree shrews through a medical purification and screening method and belongs to the technical field of clean experimental animal breeding. The method comprises following steps such as ectoparasite purification, intestinal parasite purification, salmonella and Shigella purification, Toxoplasma gondii screening and herpes virus screening; the provided method is simple and by means of medical purification and screening, the microorganism and parasite grade of experimental tree shrews is increased from normal grade to clean grade which has obvious effect. According to the method, the purification rate of ectoparasite and intestinal parasite reaches 100%, the purification rate of salmonella and Shigella reaches 97.5%; at the same time, by means of the screening method, the Toxoplasma gondii, herpes virus and pathogenic dermal fungi positives are removed; a method for breeding clean experimental tree shrews through medical purification and screening method is established. The method is initiative domestically which fills in the domestic vacancy of breeding clean experimental tree shrews.

Description

technical field [0001] The invention belongs to the technical field of cultivating clean-grade experimental animals, in particular to a method for cultivating clean-grade experimental tree shrews through drug purification and screening methods. Background technique [0002] The drug purification of experimental animals refers to the use of drugs to improve the quality level of microorganisms and parasites of the breed strains on the premise of retaining the genetic traits of the experimental animals. [0003] Existing experimental animals can be divided into four grades according to the degree of microbiological quality control or the degree of microbial purification. [0004] (1) Common-grade animals, conventional (CV) animals, do not carry the specified zoonotic pathogens and pathogens of severe animal infectious diseases. (2) Clean animals, clean (CL) animals, except for pathogens that should be excluded from ordinary animals, do not carry pathogens that are harmful to a...

AI Technical Summary

Problems solved by technology

(3) Specific pathogen-free (SPF) animals, except for pathogens that should be excluded from cleaning animals, do not carry pathogens that are major potential infections or conditionally pathogenic and greatly interfere with scientific experiments

(4) Sterile animals, germ free (GF) animal, no detectable living organisms

The general method used in other experimental animals is to take out the offspring by caesarean section under aseptic conditions to find clean experimental animals in other lactating periods as milk substitutes to provide the young animals with breast milk or artificial lactation, but in our long-term tree shrew breeding During the breeding process, these two methods have been tried for a long time, but for tree shrews, first of all, it is impossible to find suitable clean-grade milk substitute experimental animals, and secondly, there is no suitable artificial lactation formula for tree shrews, so clean-grade experiments Research on tree shrews has been blank

Images