Method for extraction of borneol from camphorwood branches and leaves and application

Abstract

Belonging to the field of traditional Chinese medicine, the invention discloses a borneol preparation method. The method consists of: washing camphorwood branches and leaves and crushing them into powder, loading the powder into a soxhlet extractor, using petroleum ether that is four times the weight of camphorwood to conduct soxhlet extraction under at a bath temperature of 90DEG C for more than 9h till the solution in a soxhlet extractor extraction cylinder has no obvious color, using a water pump to conduct pressure reduction recovery of the solvent to obtain volatile oil, then dehydrating the volatile oil with anhydrous sodium sulfate, and freezing the dehydrated volatile oil in a low temperature environment to separate out crystals, carrying out filtration, washing the crystals with ethanol, then performing dissolution with ethanol and conducting recrystallization, and then carrying out filtering and washing so as to obtain borneol. The invention also discloses a compound made of the borneol extract, and discloses application thereof in preparation of drugs controlling liver injury.

Description

technical field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a method for extracting borneol from camphor leaves. Background technique

[0002] There are multiple reasons for causing liver damage: 1. Viral infection: caused by a variety of hepatitis viruses, with strong infectivity, complex transmission routes, wide epidemic range, and high incidence rate. 2. Drugs or chemical poisons: Many drugs and chemical poisons can cause liver damage, drug-induced hepatitis or toxic hepatitis. 3. Alcoholism: Alcohol damages the liver very seriously. The consequences of the damage include alcoholic hepatitis, alcoholic fatty liver, and alcoholic cirrhosis, mainly due to the toxicity of alcohol (ethanol) and its metabolite acetaldehyde on liver cells. caused by direct damage. 4. Other reasons: primary and secondary liver tumors, liver congestion caused by cardiac insufficiency, certain congenital liver diseases, high...

Examples

Embodiment 1

[0019] Embodiment 1: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 40 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

Embodiment 2

[0020] Embodiment 2: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 60 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

Embodiment 3

[0021] Embodiment 3: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 50 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

technical field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a method for extracting borneol from camphor leaves. Background technique

[0002] There are multiple reasons for causing liver damage: 1. Viral infection: caused by a variety of hepatitis viruses, with strong infectivity, complex transmission routes, wide epidemic range, and high incidence rate. 2. Drugs or chemical poisons: Many drugs and chemical poisons can cause liver damage, drug-induced hepatitis or toxic hepatitis. 3. Alcoholism: Alcohol damages the liver very seriously. The consequences of the damage include alcoholic hepatitis, alcoholic fatty liver, and alcoholic cirrhosis, mainly due to the toxicity of alcohol (ethanol) and its metabolite acetaldehyde on liver cells. caused by direct damage. 4. Other reasons: primary and secondary liver tumors, liver congestion caused by cardiac insufficiency, certain congenital liver diseases, high...

Examples

Embodiment 1

[0019] Embodiment 1: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 40 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

Embodiment 2

[0020] Embodiment 2: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 60 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

Embodiment 3

[0021] Embodiment 3: get 10kg of camphor wood branches and leaves, wash, and be ground into powder, cross 50 mesh sieves, put into Soxhlet extractor, with the sherwood oil of 4 times of camphor wood weight, sow under the condition of 90 ℃ in bath temperature Extract for more than 9 hours, until the solution in the leaching tube of the Soxhlet extractor has no obvious color, use the water pump to depressurize the solvent to recover the solvent, and obtain the volatile oil, then dehydrate the volatile oil with anhydrous sodium sulfate and place it in a low-temperature environment to freeze , precipitate crystals, filter, wash the crystals with ethanol, then dissolve and recrystallize with ethanol, filter and wash to obtain borneol.

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