Paraffin sectioning method for eucalyptus tissue

A paraffin sectioning and tissue technology, applied in the field of plant microscopic observation, can solve the problems of hematopoiesis and nervous system poisoning, difficult to grasp the clearing time, increase the difficulty of sectioning, etc., to shorten the sealing time, reduce the fixing time, and speed up the fixing speed Effect

Inactive Publication Date: 2016-09-28
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology

However, xylene is a highly volatile and highly toxic chemical reagent, which has a strong toxic effect on human breathing, hematopoiesis and nervous system, and is also one of the carcinogens, seriously endangering the health of those who have been engaged in tissue section experiments for a long time; in a

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  • Paraffin sectioning method for eucalyptus tissue
  • Paraffin sectioning method for eucalyptus tissue

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[0043] Example 1:

[0044] (1) Acquisition and fixation

[0045] Use eucalyptus tissue culture seedlings to remove excess tissues other than the stems such as leaves, callus, etc., and take the base stems 0.5cm long. Add a polyhydroxy acrylic fixative (produced by Guangzhou Aifa Biomedical Technology Engineering Co., Ltd., the fixed speed is 1mm / h, the first type of medical treatment) into a wide-mouth glass vial with a volume of 60 times the size of the stem. Device manufacturer registration number: 20090363, stored at a temperature of 10~30℃), put in the stem, and stopper the bottle. To prevent air from entering the vial, apply petroleum jelly on the top of the rubber stopper and the contact position of the rubber stopper with the mouth of the vial. The vial is placed on the table. Insert the rubber stopper with a syringe with a needle (the piston is pushed to the bottom), but do not go deep into the fixed liquid level, and draw air. Pull out the syringe after pumping to the ma...

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Abstract

The invention belongs to the technical field of plant microscopic observation and discloses a paraffin sectioning method for eucalyptus tissue. The method comprises the steps of preparing materials, fixing, washing, dehydrating, performing a transparency treatment, waxing, embedding, sectioning, displaying the sections, adhering the sections, roasting the sections, dewaxing and rehydrating, dyeing, dehydrating, redyeing and enclosing the sections. According to the paraffin sectioning method for the eucalyptus tissue, utilized novel stationary liquid polyhydroxyl acrylic acid has high speed, can largely shorten the fixing time and can balance the fixing; meanwhile, xylene replaces with a safe and nontoxic TO transparent reagent, the TO transparent agent can be solved with alcohol of 95% at 25 DEG C or above, can solve paraffin and sealed vegetable glue, has excellent volatility without bad influence on dyeing of common biological dye, and has relatively better transparency and dewaxing effects, and after the transparency treatment is performed, the tissue is unlikely to embrittle, the sections can keep complete; furthermore, neutral vegetable glue replaces with a section sealing agent CVmount. After the fixing, dehydrating and section sealing steps during optimization of paraffin sections are performed, the section production cycle is shortened by at least 72 hours on the premise of ensuring the sectioning effect.

Description

technical field [0001] The invention belongs to the technical field of plant microscopic observation, in particular to a method for paraffin sectioning Eucalyptus (Eucalyptus) tissue. Background technique [0002] The application of paraffin section technology has a history of more than 300 years, and it is one of the most widely used methods in the routine preparation technology of plant histology. Paraffin section can not only be used to observe the morphological structure of normal plant cells and tissues, but also the main method for observing, researching and judging the morphological changes of cells and tissues in pathology and forensic science, and it has also been widely used in basic biology and other fields. research in the subject area. In recent years, with the development of biological science and technology, and the rise of plant ecological anatomy and molecular biology, paraffin section technology has been more actively used in the field of plant science res...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28G01N1/2806G01N1/286G01N2001/2873
Inventor 韩超徐建民
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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