Diluting and preserving solution for killing animal sperm viruses and bacteria

A technology for preserving fluid and semen, which can be used in the preservation of human or animal bodies, animal repellents, chemicals for biological control, etc. Benefit, improved production performance, good stability

Active Publication Date: 2016-10-12
CHENGDU MEIQIANG VETERINARY TECHNICAL SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The semen purification and protection agent in this literature is not suitable for iodine-containing disinfectants

Method used

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  • Diluting and preserving solution for killing animal sperm viruses and bacteria
  • Diluting and preserving solution for killing animal sperm viruses and bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A diluted preservation solution for killing animal semen viruses and bacteria, comprising the following components by weight: 25 parts of glucose, 2.5 parts of citric acid, 5 parts of trisodium citrate, 1.2 parts of sodium bicarbonate, disodium edetate 1.2 parts, 0.7 parts of potassium chloride, 2.8 parts of tris, 0.05 parts of cysteine ​​and 0.025 parts of povidone-iodine.

Embodiment 2

[0032] A diluted preservation solution for killing animal semen viruses and bacteria, comprising the following components by weight: 40 parts of glucose, 3 parts of citric acid, 8 parts of trisodium citrate, 1.3 parts of sodium bicarbonate, disodium edetate 1.3 parts, 0.9 parts of potassium chloride, 3 parts of tris, 0.08 parts of cysteine, 0.030 parts of povidone-iodine and 0.002 parts of antimicrobial peptide.

[0033] The pH value of the diluted preservation solution is 6.2, and the osmotic pressure is 347 mOsm.

[0034] The solvent of the diluted preservation solution is double distilled water.

Embodiment 3

[0036]A diluted preservation solution for killing animal semen viruses and bacteria, comprising the following components by weight: 30 parts of glucose, 2.6 parts of citric acid, 6 parts of trisodium citrate, 1.22 parts of sodium bicarbonate, disodium edetate 1.22 parts, 0.8 parts of potassium chloride, 2.9 parts of tris, 0.06 parts of cysteine ​​and 0.026 parts of povidone-iodine.

[0037] The pH value of the diluted preservation solution is 6.3, and the osmotic pressure is 347 mOsm.

[0038] The solvent of the diluted preservation solution is double distilled water.

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Abstract

The invention provides a diluting and preserving solution for killing animal sperm viruses and bacteria. The solution is prepared from, by weight, 25-40 parts of glucose, 2.5-3 parts of citric acid, 5-8 parts of trisodium citrate, 1.2-1.3 parts of sodium bicarbonate, 1.2-1.3 parts of ethylenediaminetetraacetic acid disodium salt, 0.7-0.9 part of potassium chloride, 2.8-3 parts of trihydroxymethyl aminomethane, 0.05-0.08 part of cysteine, 0.025-0.030 part of povidone-iodine and 0-0.002 part of antibacterial peptide. The diluting and preserving solution is used for dilution, preservation and bacteria and virus killing of animal sperm.

Description

technical field [0001] The invention belongs to the field of artificial insemination of animals in animal husbandry, and in particular relates to a diluted preservation solution for killing virus and bacteria in animal semen. Background technique [0002] The advantage of artificial insemination technology is that it can collect high-quality animal semen manually, and through certain technical means, it can be stored and transported for a long time, which will bring huge economic benefits to the improvement of animal husbandry varieties and production performance. However, artificial insemination technology also has its natural defect, that is, it can infect the recipient with all the pathogenic microorganisms that may be contained in the donor's semen. Taking pigs as an example, boar semen usually contains 10 4 ~10 5 CFU / ml of bacteria and possibly contaminated unknown viruses and other pathogenic microorganisms. These bacteria and viruses will affect the storage time o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A01N63/02A01P1/00A01N43/16A01N37/36A01N59/00A01N37/44A01N59/08A01N59/12
CPCA01N1/0215A01N37/36A01N37/44A01N43/16A01N59/00A01N59/08A01N59/12A01N63/10A01N2300/00
Inventor 况洪
Owner CHENGDU MEIQIANG VETERINARY TECHNICAL SERVICE CO LTD
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