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Frozen section embedding agent

A technology of frozen section and embedding agent, which is applied in the field of in vitro diagnostic reagents, which can solve the problems of high price, inconsistency, and difficulty in meeting technical requirements, and achieve the effect of preventing deterioration and corruption, preventing slice deterioration, and good bactericidal effect

Inactive Publication Date: 2016-10-12
北京九州柏林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the embedding agents used in hospitals are all imported products, and the indicators are not good or bad, and the price is expensive. Due to the price problem, paste is used instead of embedding agent. This is not a solution to the problem, and its efficacy is difficult to meet the technical requirements.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An embedding agent for frozen sections, comprising:

[0023] Purified water 1000g, polyvinyl alcohol 124 80g, polyvinylpyrrolidone 30g, sodium carboxymethylcellulose 10g and polyethylene glycol 300 50g.

[0024] Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethylcellulose and polyethylene glycol 300 were all analytically pure.

Embodiment 2

[0026] An embedding agent for frozen sections, comprising:

[0027] Purified water 1000g, polyvinyl alcohol 124 100g, polyvinylpyrrolidone 50g, sodium carboxymethylcellulose 20g and polyethylene glycol 300 80g.

[0028] Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethylcellulose and polyethylene glycol 300 were all analytically pure.

Embodiment 3

[0030] An embedding agent for frozen sections, comprising:

[0031] 1000 parts of purified water, 90 g of polyvinyl alcohol 124, 40 g of polyvinyl pyrrolidone, 15 g of sodium carboxymethyl cellulose and 65 g of polyethylene glycol 300.

[0032] Polyvinyl alcohol 124, polyvinylpyrrolidone, sodium carboxymethylcellulose and polyethylene glycol 300 were all analytically pure.

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PUM

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Abstract

The invention discloses a frozen section embedding agent, which comprises the following components in parts by mass: 1000 parts of purified water, 80-100 parts of polyvinyl alcohol, 30-50 parts of polyvinylpyrrolidone, sodium carboxymethyl cellulose 10-20 parts and polyethylene glycol 300 50-80 parts. The embedding agent designed in the present invention has good thermal conductivity, transparency, viscosity, water solubility and ductility, and its hardness is suitable. The frozen section embedding agent of the present invention can be applied to the embedding of tissue for pathological diagnosis in surgery, and can also be used for conventional pathological diagnosis, immunohistochemical diagnosis technology and molecular biology diagnosis technology.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, in particular to a frozen section embedding agent. Background technique [0002] Routine pathological diagnosis is that the human tissue removed by the surgeon is sent to the pathology department, and the pathological technician prepares the tissue specimen (fixed, dehydrated, transparent, and soaked in wax); embedding, slicing, and baking; staining (dewaxing, After hematoxylin dyeing, hematoxylin staining, differentiation, bluing, eosin staining, transparency, and sealing), the stained slices are sent to the pathologist, who observes the slices through a microscope to make a pathological diagnosis of the patient's disease . This process generally takes about 3 days, so the conventional pathological diagnosis seems to lag behind the surgeon's surgical treatment, which may cause a second surgical treatment for the patient and cause unnecessary pain to the patient. In order to synchronize patholo...

Claims

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Application Information

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IPC IPC(8): G01N1/36
CPCG01N1/36
Inventor 李明王德田
Owner 北京九州柏林生物科技有限公司
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