Vibrio medium for detecting content of vibrio in aquaculture water

A technology of aquaculture and culture medium, which is applied in the direction of microorganism-based method, measurement/inspection of microorganisms, microorganisms, etc., can solve the problems of serious discrepancy between the number of Vibrio detection and the actual value, high detection rate of Vibrio, etc., and achieve preventive technology The effect of high value and detection rate

Inactive Publication Date: 2016-11-02
SHANGHAI LYUAO BIOTECH CO LTD +1
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Problems solved by technology

The invention discloses a vibrio medium for detecting the content of vibrio in aquaculture water, which has a high detection rate of vibrio, can accurately reflect the total amount of vibrio in the aquaculture water, and avoids the impact of the TCBS medium on the vibrio in the aquaculture process. The phenomenon that the number of vibrio is seriously inconsistent with the actual value has important technical value for preventing the occurrence of vibriosis in aquaculture

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  • Vibrio medium for detecting content of vibrio in aquaculture water

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Embodiment Construction

[0008] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0009] 1. Experimental materials

[0010] Glutamic acid, tryptone, sodium thiosulfate, glyceryl monolaurate, lactose, sodium chloride, ferric ammonium citrate, thymol blue, agar powder, distilled water, cultured water of Penaeus vannamei, cultured water of crucian carp, loach Breeding water, sterile petri dish, sterile water, TCBS medium plate.

[0011] 2. Experimental method

[0012] 1. Components of Vibrio culture medium.

[0013] Mix glutamic acid, tryptone, sodium thiosulfate, glyceryl monolaurate, lactose, sodium chloride, ferric ammonium citrate, thymol blue, agar powder, and distilled water, heat and boil, then pour into a sterile petri dish to solidify .

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Abstract

The invention discloses a vibrio medium for detecting the content of vibrio in aquaculture water. Specifically, the vibrio medium comprises 2.0g of glutamic acid, 15.0g of tryptone, 15.0g of sodium thiosulfate, 5.0g of glycerol monolaurate, 20.0g of lactose, 15.0g of sodium chloride, 1.5g of ammonium ferric citrate, 0.04g of thymol blue, 20.0g of agar powder, and 1000ml of distilled water, and has a pH value of 7.5. The components are mixed, and heated to boiling for 35min, then the product is poured into 22ml/dish culture dish, and after solidification, the medium can be used for vibrio detection. The vibrio medium for detecting the content of vibrio in aquaculture water provided by the invention has high vibrio detection rate, can accurately reflect the total amount of vibrios in culture water, has a vibrio detection rate significantly superior to that of a TCBS medium, avoids the phenomenon of serious discrepancy between vibrio amount detection by a TCBS medium and an actual value during culture, and has important technological value for prevention of aquaculture vibriosis.

Description

technical field [0001] The invention relates to a Vibrio culture medium, in particular to a Vibrio culture medium for detecting Vibrio content in aquaculture water. Background technique [0002] Vibrio is a Gram-negative bacterium that widely exists in aquaculture water bodies and is an important pathogen that causes diseases in aquaculture fish, shrimp, and shellfish. Vibrio is easy to reproduce in large numbers when the residual bait and feces increase, the water quality and bottom quality deteriorate, and the water temperature is high. When the number of Vibrio exceeds the threshold, it is easy to cause the outbreak and rapid spread of vibriosis in aquaculture animals. Therefore, it is of great significance to accurately count the total amount of Vibrio in aquaculture water for healthy aquaculture. At present, farmers widely use TCBS medium for Vibrio enumeration in aquaculture. However, although TCBS medium can cultivate Vibrio in aquaculture water, other bacteria can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12R1/63
Inventor 曹海鹏李家胜
Owner SHANGHAI LYUAO BIOTECH CO LTD
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