Aspergillus sp. for producing anti-HIV active substance and application of aspergillus sp.
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A microbial strain, the technology of Aspergillus, applied in the field of Aspergillus, can solve the problems of drug resistance, adverse reaction patient compliance, clinical treatment failure, etc.
Active Publication Date: 2016-11-09
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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With the continuous development and application of anti-HIV drugs, especially the treatment of AIDS patients relying on combined drug therapy, it can effectively reduce the viral load and significantly prolong the survival time of patients, but drug resistance, adverse reactions and patient compliance, etc. Problems still often cause failure of clinical treatment
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Embodiment 1
[0090] Example 1. Isolation and Identification of Aspergillus sp. CPCC400735
[0091] 1.1 Strain isolation
[0092] Strain CPCC400735 was isolated from the stem of Kadsura longiped unculata. Collect the plants of Schisandra chinensis, put them into ziplock bags, and bring them back to the laboratory for processing. Weigh 1g of the stem of Schisandra chinensis, rinse it with 75% alcohol for 30s, wash it with sterile water for 3 times, cut the stem into pieces with sterile scissors, put it into a sterilized mortar and add quartz sand for grinding. Add the grinding solution to a centrifuge tube filled with 9 mL of sterile water, and the sample concentration is 10 -1 , shake well, and then make 10 -2 、10 -3 、10 -4 、10 -5 Gradient dilution. Draw 200 μL of different gradient dilutions, spread them on PDA plates, blow dry, and place them upside down in an incubator at 25°C for 3-5 days. Transfer the isolated fungi to the PDA slant for culture. After they grow well, Store in t...
[0100] This embodiment utilizes Aspergillus sp. (Aspergillus sp.) CPCC400735 to prepare 5 kinds of HIV-1 inhibitors, one of which is the methanol extract of Aspergillus sp. (Aspergillus sp.) CPCC400735 fermentation culture (from Aspergillus sp. (Aspergillus sp.) The substance dissolved in methanol extracted from the culture of CPCC400735), and the other four HIV-1 inhibitors are the general formula compounds shown in formula II
[0101] Formula II, its structural formula is specifically shown in formula 1 to formula 4 (Table 1).
[0102] The substituents of the general formula compound shown in table 1, formula II
[0103]
[0104] The concrete preparation method of above-mentioned 5 kinds of HIV-1 inhibitors is as follows:
[0105] 1. Prepare the culture of Aspergillus sp. CPCC400735
[0106] Aspergillus sp. (Aspergillus sp.) CPCC400735 was cultivated for 5 days at 2...
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Abstract
The invention discloses an aspergillus sp. for producing an anti-HIV active substance and application of the aspergillus sp.. The strain number of the aspergillus sp. is CPCC400735, and the registration number of the aspergillus sp. in Center of General Microbiology of China Committee for Culture Collection of Microorganisms is CGMCC No.12376. The inhibition ratio of methyl alcohol extractive of fermentation culture of the aspergillus sp. CPCC400735 to HIV-1 is 99.9% when the concentration is 4 mg/mL. The inhibition ratio of a compound in a formula 1, the inhibition ratio of a compound in a formula 2, the inhibition ratio of a compound in a formula 3 and the inhibition ratio of a compound in a formula 4 to HIV-1 are 96% or above when the concentration is 100 micromol/L, and the IC50 value is 2.4-32.6 micromol/L. The methyl alcohol extractive of the fermentation culture of the aspergillus sp. CPCC400735, the compound in the formula 1, the compound in the formula 2, the compound in the formula 3 and the compound in the formula 4 have high inhibitory activity to HIV-1 and have less toxicity.
Description
technical field [0001] The invention relates to an aspergillus strain producing anti-HIV active substances and its application. Background technique [0002] HIV (Human Immunodeficiency Virus) virus, the full name of human immunodeficiency virus in Chinese, is a kind of retrovirus. After the HIV virus invades the host cells, it will severely damage the immune function of the host body, thereby causing severe opportunistic infections and causing secondary immunodeficiency characterized by malignant diseases, which is the acquired immunodeficiency syndrome (AIDS). ). The first case of AIDS was discovered in the United States in 1981, and then began to spread rapidly around the world, and has become one of the major threats to human health. HIV virus can be divided into HIV-1 type and HIV-2 type, and the genomes of the two have great differences, among which HIV-1 is the main pathogen that causes the spread of global AIDS. [0003] Chemotherapy is still the most important tr...
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