A culture solution for promoting rapid growth of microorganisms and its application method

A culture medium and rapid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long time consumption and low efficiency, and achieve the effect of high efficiency, clean environment and short time consumption
CN106085944BActive Publication Date: 2019-07-30SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2019-07-30

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Abstract

The invention discloses a culture solution for accelerating rapid growth of microorganisms and a using method thereof. Bacillus pasteurii is inoculated into a culture medium containing various nutrient substances, constant temperature shaking culture is performed at 25-30 DEG C, the initial PH value is controlled to be about 6, culture is performed for 12-24 h, part of bacteria solution is taken out every two days in the culture process, a spectrophotometer is utilized to measure the absorbance OD600, a strain growth curve is obtained, strains enter into a stable stage in about 18 h according to observation, and compared with research by predecessors, the strain growth speed is obviously accelerated. The culture solution for accelerating rapid growth of the microorganisms and the using method have the advantages that substances in the culture solution can be fully utilized, and the culture solution is simple in process, high in efficiency, short in used time, clean in environment and low in cost; the efficiency of microbial induction of calcium carbonate is further improved, and the concrete crack repair time can be shortened.
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Description

technical field

[0001] The invention relates to a preparation method of a culture solution, in particular to a preparation method of a culture solution for promoting rapid growth of microorganisms. Background technique

[0002] Biomineralization is a very common natural phenomenon, and almost every kind of organism can synthesize minerals. Nearly two-thirds of it is calcium ore, and a considerable part has a cementing function. Through their own life activities, they are constantly enzymatically reacting with the surrounding environment medium to gradually form calcium carbonate. Using microorganisms to prepare calcium carbonate has the advantages of clean environment, green environment, simple process and low cost, and this technology has been widely used. At the same time, Bacillus pasteurianus is a commonly used bacterium for preparing calcium carbonate, but because it takes nearly 48 hours to reach the stable period under general culture conditions, it is time-consumin...

Claims

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