Method for Establishing Fingerprint of Daluosan Medicinal Material and Its Fingerprint

A technology of fingerprint spectrum and establishment method, which is applied to the establishment method of the fingerprint spectrum of D. auratus medicinal materials and the field of fingerprints, can solve the problem of weak control of the quality of D. grandiflorum medicinal materials, lack of a quality evaluation system, and it is difficult to achieve effective quality of D. grandiflorum. Control and other issues to achieve the effect of ensuring products and improving the quality evaluation system

Active Publication Date: 2018-04-03
GUANGXI UNIVERSITY OF TECHNOLOGY
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AI Technical Summary

Problems solved by technology

However, there is no quality standard for the different parts of the medicinal material of Daluosan at present, and the quality control of the medicinal material of Daluosan is very weak. It only includes routine inspections such as microscopic identification, and lacks a perfect quality evaluation system. Effective Control of Quality

Method used

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  • Method for Establishing Fingerprint of Daluosan Medicinal Material and Its Fingerprint
  • Method for Establishing Fingerprint of Daluosan Medicinal Material and Its Fingerprint
  • Method for Establishing Fingerprint of Daluosan Medicinal Material and Its Fingerprint

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Experimental program
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Effect test

preparation example Construction

[0064] 2.1 Selection of preparation conditions for the test product

[0065] 2.1.1, Selection of extraction solvent

[0066] Precisely weigh 3 parts of No. 5 Daluosan herbal medicine, 2.0g each, and place them in colorimetric tubes, respectively add 30% methanol, 60% methanol and pure methanol solution as extractants, and ultrasonically Treat for 40 minutes, let cool, weigh again, use 30% methanol, 60% methanol and pure methanol solution to make up the lost weight, shake well, filter to obtain the test solution. Under the conditions of the same detection method, measure its fingerprints respectively, and determine the best extractant by comparison; as figure 1 , figure 2 and image 3 Shown, under the same situation of other conditions, respectively use 30% methanol, 60% methanol and pure methanol to extract the chromatogram comparison of the test sample prepared by the cinnabar root of the same quality, the extractant is the cinnabar root spectrum of 60% methanol The peak...

Embodiment 1

[0081] Establish Daluosan Medicinal Materials

[0082] (1) Preparation of the test solution:

[0083] Accurately weigh 3.0g of Daluosan herbal powder and place it in a colorimetric tube, add 9mL of 60% methanol precisely, soak for 0.5h, ultrasonicate for 40min, let cool, make up the lost weight with 60% methanol solution, shake well, filter After that, the test solution was obtained;

[0084] (2) HPLC detection:

[0085] Draw the test solution obtained in the above step (1), inject it into a high-performance liquid chromatograph for high-performance liquid chromatography detection, and record the fingerprint within 50 min:

[0086] Wherein, the fingerprint chromatographic conditions are:

[0087] Adopt American AgiLent XDB-C18 chromatographic column (4.6×150mm, 5μm), mobile phase is acetonitrile-water system; adopt gradient elution method: 0min→10min→20min→30min→40min→50min, acetonitrile 45%→50%→55 %→55%→60%→63%; the detection wavelength is 280nm, the flow rate is 0.8mL / mi...

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Abstract

The present invention discloses a method for establishing the fingerprints of medicinal materials of Daluosan and its fingerprints, and relates to the technical field of fingerprints of traditional Chinese medicinal materials. The method includes the preparation of a test solution and the detection conditions of high performance liquid chromatography. Place in a test tube, add 60% methanol solution, ultrasonically shake for 40min, let cool, weigh again, make up the lost weight with 60% methanol solution, shake well, filter, and the filtrate is used as the test solution; for the test The product solution was detected by high-performance liquid chromatography, using AgiLent XDB-C18 chromatographic column, acetonitrile-water system mobile phase; using gradient elution; controlling the detection wavelength, column temperature, flow rate and analysis time, and obtained the fingerprint of Daluosan medicinal material. The fingerprint chromatogram of the medicinal material of Daluo umbrella established by the invention can effectively monitor the quality of different medicinal parts of the medicinal material of Daluo umbrella and identify its authenticity.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing the fingerprints of Daluosan medicinal materials and the fingerprints thereof. Background technique [0002] Although traditional Chinese medicine has a history of thousands of years of application and development, how to effectively evaluate the quality of Chinese medicinal materials has always been the focus and difficulty of research and application of Chinese medicinal materials. With the development of science and technology, fingerprint quality control technology has been proposed in the field of traditional Chinese medicine and has been widely recognized, and its application in the quality control of traditional Chinese medicine is also increasing; The atlas that can indicate the characteristics of the Chinese herbal medicine obtained by certain analytical means and testing instruments is one of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 黄琼黄永春杨锋任仙娥唐福宇
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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