Preparation method of agaricus bisporus liquid strain
A technology of liquid strains and Agaricus bisporus, which is applied in the field of preparation of liquid strains of Agaricus bisporus, can solve the problems of easy breeding of miscellaneous bacteria and unreasonable medium ratio, etc., and achieve the effects of enhancing the vitality of the strains, shortening the growth cycle, and improving the quality
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Embodiment 1
[0028] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:
[0029] (1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 5 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2cm;
[0030] The slant culture medium is prepared by adding 8 mL of green tea extract, 25 g of cornstarch, 2 g of agar, 0.5 g of casein hydrolyzed peptide, 0.8 g of peptone, and 0.35 g of potassium dihydrogen phosphate per 1000 mL of purified water, stirring evenly, and cooling after sterilization ;
[0031] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture them on a shaking table at a temperature of 20-22°C for 2 days, and then culture them statically at 20-22°C for 1 day to obtain primary culture medium;
[0032] Add 20mL of green tea extract, 10g of peptone, 50g of ...
Embodiment 2
[0038] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:
[0039] (1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 6 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2 cm;
[0040] The slant culture medium is prepared by adding 9 mL of green tea extract, 20 g of cornstarch, 1 g of agar, 0.3 g of casein hydrolyzed peptide, 1.0 g of peptone, and 0.5 g of potassium dihydrogen phosphate for every 1000 mL of purified water, stirring evenly, and cooling after sterilization ;
[0041] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture it on a shaking table at a temperature of 20-22°C for 1 day, and then culture it statically at 20-22°C for 2 days to obtain primary culture medium;
[0042] 15mL of green tea extract, 9g of peptone, 53g of sol...
Embodiment 3
[0048] A preparation method of Agaricus bisporus liquid spawn, comprising the following steps:
[0049](1) Inoculate the mother species of Agaricus bisporus on the first-level slant medium, and cultivate it at 24-26°C for 5 days to obtain the slant strains of Agaricus bisporus, which are divided into slant strain blocks with a growth width of 2cm;
[0050] The slant culture medium is prepared by adding 10mL green tea extract, 28g cornstarch, 2g agar, 0.2g casein hydrolyzate peptide, 1.2g peptone, 0.2g potassium dihydrogen phosphate to every 1000mL purified water, stir evenly, and cool down after sterilization ;
[0051] (2) Inoculate 5-6 pieces of first-grade Pleurotus eryngii slant strains into the first-grade culture medium, culture them on a shaking table at a temperature of 20-22°C for 2 days, and then culture them statically at 20-22°C for 1 day to obtain primary culture medium;
[0052] Add 18mL of green tea extract, 7g of peptone, 55g of soluble starch, 8g of glycerol...
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