Method for optimizing recipe for extracting compound Xuezhining from beta-cyclodextrin based on fingerprint spectrum technique

A fingerprint, Xuezhining technology is applied in the field of optimizing the prescription of β-cyclodextrin extraction compound Xuezhining based on the fingerprint technology, and achieves the effects of ensuring consistency, reducing industrial production costs, and ensuring drug efficacy.

Inactive Publication Date: 2016-11-23
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0004] The present invention has designed and developed a method for optimizing the prescription of compound Xuezhining extracted by β-cyclodextrin based on fingerprint technology, and the purpose of the invention is to solve the problem of extracting compound Xuezhining medicinal material by using β-cyclodextrin to ensure that the proportion and content of the extracted components are consistent Reduce the amount of medicinal materials in the prescription under certain circumstances, and obtain the problem of the optimized prescription amount

Method used

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  • Method for optimizing recipe for extracting compound Xuezhining from beta-cyclodextrin based on fingerprint spectrum technique
  • Method for optimizing recipe for extracting compound Xuezhining from beta-cyclodextrin based on fingerprint spectrum technique
  • Method for optimizing recipe for extracting compound Xuezhining from beta-cyclodextrin based on fingerprint spectrum technique

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Experimental program
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Embodiment

[0054] 1 material

[0055] 1.1 Drugs

[0056] Cassia (origin: Vietnam), Zhishouwu (origin: Sichuan), lotus leaf (origin: Shandong), hawthorn (origin: Chengde).

[0057] 1.2 Reagents

[0058]

[0059] 1.3 Instruments

[0060]

[0061] 2 Methods and results

[0062] 2.1 Fingerprint of Compound Xuezhining Traditional Chinese Medicine

[0063] 2.1.1 Chromatographic conditions

[0064] Chromatographic column: Acquity UPLC BEH shield RP18 (2.1×100mm, 1.7μm); mobile phase: methanol (A)-0.1% formic acid aqueous solution (B), the gradient elution program is shown in the table below; flow rate: 0.2mL / min; column temperature : 45°C; detection wavelength: 280nm; analysis time: 40min; injection volume 3μL; Figure 1~3 Shown is the high performance liquid chromatogram of Xuezhining.

[0065] Table 1 Gradient elution table

[0066]

[0067] 2.1.2 Preparation of sample solution

[0068] Xuezhining traditional water extraction process

[0069] According to the compound recip...

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Abstract

The invention discloses a method for optimizing a recipe for extracting compound Xuezhining from beta-cyclodextrin based on a fingerprint spectrum technique. The method comprises the following steps: (1) selecting multiple recipe amounts of compound Xuezhining, and respectively preparing a Xuezhining water extract and a Xuezhining beta-cyclodextrin extract; (2) respectively establishing fingerprint spectrums of the uezhining water extract and the Xuezhining beta-cyclodextrin extract, and selecting multiple common fingerprint peaks; (3) carrying out medical material affiliation on the common fingerprint peaks, and calculating a corresponding chromatographic peak area content formula (shown in the description) according to chromatographic peak areas in the common fingerprint peaks; and (4) respectively calculating the minimum values of the chromatographic peak similarities formula (shown in the description) in different medical materials after the optimization according to the chromatographic peak areas of the common fingerprint peaks in the Xuezhining water extract and the peak area content of the common fingerprint peak in the Xuezhining beta-cyclodextrin extract, so as to determine the dosage of each medical material in a final optimized compound Xuezhining recipe.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extraction process, in particular to a method for optimizing the prescription of compound Xuezhining extracted from β-cyclodextrin based on fingerprint technology. Background technique [0002] The traditional Chinese medicine compound Xuezhining is recorded in the 2010 edition of "The Pharmacopoeia of the People's Republic of China". It is composed of four traditional Chinese medicines: cassia seed, shouwu, lotus leaf, and hawthorn. Hyperlipidemia due to stasis. Cyclodextrin (CD for short) and its derivatives are new types of medicinal materials that have developed rapidly in recent years, and have the characteristics of low biological toxicity and good water solubility. β-cyclodextrin (β-CD) is a cyclic oligomer linked by α-1,4-glucosidic bonds obtained from starch fermented by glucosyltransferase, which contains 7 glucopyranose units. With a special molecular structure of internal hydrophobic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
CPCG01N30/86G01N30/8686
Inventor 张慧杰任晓亮王萌孙立丽刘亚男邱喜龙戚爱棣
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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