A kind of Acetobacter pasteurian with excellent heat resistance and application thereof

Abstract

The invention discloses a kind of Acetobacter pasteurii with excellent heat resistance Acetobacer pasteurianus DY4 and its uses, the strains are preserved at the China Center for Type Culture Collection (Wuhan University, Wuhan, China), and the preservation number is: CCTCC NO: M 2015462. Under the condition of 4% ethanol, the Acetobacter pasteuriani described in the present invention can have relatively stable growth and acid production (ethanol-acetic acid conversion rate>90.6%) at 30-40°C, and reach the maximum conversion rate (97.5% at 37°C). %), OD at high temperature of 43℃ 600 It can reach 0.72, and the acid production can reach 16.5g / L (the maximum conversion rate is 40.0%); under the condition of 37°C and 8% ethanol, the ethanol conversion rate can reach 95.6%. Therefore, the bacterial strain of the invention is suitable for brewing fruit vinegar in summer, and the flavor of the vinegar product is superior.

Description

technical field [0001] The invention belongs to the field of microorganism and fermentation industry, and in particular relates to a kind of Acetobacter pasteurii with excellent heat resistance Acetobacer pasteurianus DY4 and its uses. Background technique [0002] Acetic acid bacteria (AAB) are obligate aerobic Gram-negative bacteria capable of oxidizing acetic acid from ethanol (Deppenmeier et al. 2010). AAB is commonly isolated from natural habitats and from vinegar fermented grains (Wu et al. 2010). During the fermentation process, acetic acid bacteria will be subjected to various stresses, such as high temperature. The optimum growth temperature for AAB is 25- [0003] 30°C, although acetic acid bacteria can also grow at 37-40°C (Gullo et al. 2008), but the growth is slow. The heat of fermentation accumulated during the fermentation of acetic acid bacteria necessarily makes the fermentation temperature higher than 37 °C, resulting in slow cell growth and low acetic...

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Problems solved by technology

[0003] 30°C, although acetic acid bacteria can also grow at 37-40°C (Gullo et al.2008), but the growth is slow

In summer, the temperature is high, and the commonly used acetic acid bacteria AS1.41 and Huniang 1.01 are difficult to withstand high temperature, and the output of acetic acid is not high. The production of the above fruit vinegars requires high cooling costs. too expensive

In order to improve the level of acetic acid fermentation under high-temperature conditions, the improvement of the heat-resistant function of acetic acid bacteria has become a research hotspot, such as the Chinese patent application number CN201310549668, "High-temperature-resistant acetic acid bacteria and its application in the production of acetic acid by fermentation", in which the acetic acid Bacteria can grow at 45°C (high temperature resistance)), the ethanol-acetic acid conversion rate at 37°C and 7% ethanol is 88.8%, and the ethanol-acetic acid conversion rate at 37°C and cider (7% ethanol) is 93% %, there is no report of acetic acid bacteria with superior high temperature resistance

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