Method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and multivariate statistical technique

A multi-variable statistical and miniature seed potato technology, applied in the field of plant analytical chemistry, can solve the problems of difficult to accurately detect active seeds and large environmental impact, and achieve high sensitivity, fast analysis speed and good stability

Inactive Publication Date: 2016-12-07
BAOJI UNIV OF ARTS & SCI
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Problems solved by technology

However, some experiments have shown that the conductivity method is difficult to accurately detect seeds with too low activity, and the seed activity can only be evaluated indirectly through the amount of released electrolytes, and the results are greatly affected by the environment

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  • Method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and multivariate statistical technique
  • Method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and multivariate statistical technique
  • Method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and multivariate statistical technique

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Embodiment Construction

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0028] The sample to be tested used in the present invention is potato miniature seed potato, which was harvested in 2016 and provided by the Dingxi Dry Farming Agricultural Research and Promotion Center in Gansu Province. Cultivate miniature seed potatoes in stone.

[0029]Instruments, reagents and materials used in the present invention are: linear ion trap mass spectrometer (LTQ-XL type of U.S. Finnigan company), equipped with LTQ Xcalibur 2.0 software proc...

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Abstract

The invention provides a method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and a multivariate statistical technique. The method comprises steps as follows: to-be-tested mini-sized potato seed samples are subjected to artificial aging acceleration processing; the mini-sized potato seeds after artificial aging acceleration treatment are subjected to a germination standard test, and germination indexes and activity indexes are calculated; the mini-sized potato seeds subjected to aging acceleration treatment are selected through random sampling for preparation of extracting solutions, and supernatants are obtained; the supernatants of the mini-sized potato seed extracting solutions are subjected to EESI-MS analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with a variable analysis method. According to the method, the mini-sized potato seed extracting solutions with different activity levels are subjected to mass spectrometric analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with the variable analysis method, so that the method has the advantages of being good in stability, high in sensitivity, high in analysis speed and the like.

Description

technical field [0001] The invention relates to the technical field of plant analytical chemistry, in particular to a method for identifying the activity of potato micro-seed tubers based on EESI-MS and multivariate statistical techniques. Background technique [0002] Potato plays an important role in my country's agricultural production and planting structure adjustment, especially in the arid and semi-arid areas of Northwest China and the alpine mountainous areas of Southwest China. Potatoes are not only the main food crops, but also important economic crops. Its important role in development cannot be underestimated. Since the 1980s, with the reform and opening up of my country's economy, potato production has developed greatly. The planting area has increased from 2.45 million hm in 1982 2 Developed to 8.7 million hm in 2012 2 , the total output increased from 23.83 million tons to 75 million tons, and the unit yield increased from 9.7 tons / hm 2 Increased to 16 tons / ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/02
CPCA01C1/00A01C1/025
Inventor 王乐杨得锁王晓玲谈娜娜
Owner BAOJI UNIV OF ARTS & SCI
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