Method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and multivariate statistical technique

Abstract

The invention provides a method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and a multivariate statistical technique. The method comprises steps as follows: to-be-tested mini-sized potato seed samples are subjected to artificial aging acceleration processing; the mini-sized potato seeds after artificial aging acceleration treatment are subjected to a germination standard test, and germination indexes and activity indexes are calculated; the mini-sized potato seeds subjected to aging acceleration treatment are selected through random sampling for preparation of extracting solutions, and supernatants are obtained; the supernatants of the mini-sized potato seed extracting solutions are subjected to EESI-MS analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with a variable analysis method. According to the method, the mini-sized potato seed extracting solutions with different activity levels are subjected to mass spectrometric analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with the variable analysis method, so that the method has the advantages of being good in stability, high in sensitivity, high in analysis speed and the like.

Description

technical field [0001] The invention relates to the technical field of plant analytical chemistry, in particular to a method for identifying the activity of potato micro-seed tubers based on EESI-MS and multivariate statistical techniques. Background technique [0002] Potato plays an important role in my country's agricultural production and planting structure adjustment, especially in the arid and semi-arid areas of Northwest China and the alpine mountainous areas of Southwest China. Potatoes are not only the main food crops, but also important economic crops. Its important role in development cannot be underestimated. Since the 1980s, with the reform and opening up of my country's economy, potato production has developed greatly. The planting area has increased from 2.45 million hm in 1982 2 Developed to 8.7 million hm in 2012 2 , the total output increased from 23.83 million tons to 75 million tons, and the unit yield increased from 9.7 tons / hm 2 Increased to 16 tons / ...

AI Technical Summary

Problems solved by technology

However, some experiments have shown that the conductivity method is difficult to accurately detect seeds with too low activity, and the seed activity can only be evaluated indirectly through the amount of released electrolytes, and the results are greatly affected by the environment

Images