Method for improving immune effect of newcastle disease inactivated vaccine by utilizing adenovirus for expressing GM-CSF (granulocyte macrophagecolony stimulating factor) and kit

Abstract

The invention relates to the technical field of microorganisms and biology, in particular to a method for improving an immune effect of newcastle disease inactivated vaccine by utilizing an adenovirus for expressing a GM-CSF (granulocyte macrophagecolony stimulating factor), and a kit. The method comprises the following steps: uniformly mixing a recombinant adenovirus for expressing the GM-CSF and the newcastle disease inactivated vaccine to obtain a vaccine immunity adjuvant mixture; then, performing immunization on poultry by utilizing the vaccine immunity adjuvant mixture. The invention also relates to an immunity adjuvant which is formed by the recombinant adenovirus for expressing the GM-CSF, and the kit which consists the recombinant adenovirus for expressing the GM-CSF and the newcastle disease inactivated vaccine. The method and the kit can be used for the poultry culturing industry.

Description

technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a method and a kit for improving the immune effect of an inactivated Newcastle disease vaccine by using an adenovirus expressing GM-CSF. Background technique [0002] Newcastle Disease (ND) is an acute, highly contagious disease of poultry such as chickens, turkeys, and pigeons caused by Newcastle disease virus. It mainly causes the death of poultry by causing sepsis. One of the three major infectious diseases in China, this disease has caused great harm in many countries and regions in Asia for many years. At present, the most effective way to deal with Newcastle disease is to inject Newcastle disease inactivated vaccine, which can effectively prevent the occurrence and spread of poultry Newcastle disease. [0003] Immune adjuvants, also known as non-specific immune proliferators, are non-specific immune enhancers that are not antigenic in themselves, but can eff...

AI Technical Summary

Problems solved by technology

[0007] The problem to be solved in the present invention is: the purpose of the present invention is to overcome the problem that the eukaryotic or prokaryotic expression system is used as the delivery carrier when GM-CSF is used as an immune adjuvant because the half-life of GM-CSF is short and cannot be well Play the role of immune adjuvant and greatly affect the technical issues of its immune enhancement effect

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