Stabilizing agent for uric-acid detection reagent

Abstract

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a stabilizing agent for a uric-acid detection reagent. The stabilizing agent is characterized by being prepared from the following components in parts by weight: 0.1-5g / L of MgSO4, 1-5ml / L of glycerinum, 0.1-20g / L of trehalose, 0.1-10ml / L of PEG (Polyethylene Glycol) 4000, 0.1-5g / L of adenosine diphosphate, 1-4g / L of alpha-oxoglutarate, 0.5-2g / L of polyvinylpyrrolidone and 0.5-2.0g / L of polyethyleneimine. The stabilizing agent for the uric-acid detection reagent has the advantages that the effective period of the uric-acid detection reagent can be obviously prolonged to 2 years, the stability after opening lasts for 3 months, a blank absorbency value is obviously reduced, single-reagent operation is simple, the result is accurate, and the stability is good.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a stabilizer for uric acid detection reagents with simple configuration, extended reagent validity period and increased bottle-opening stability. Background technique [0002] As we all know, in vitro determination of uric acid (UA) content in human serum or plasma is one of the important indicators of renal function. Uric acid is the final product of purine catabolism in the human body, which is excreted by the kidneys with urine. When the kidney function is severely damaged, the uric acid in the blood increases significantly; when suffering from hereditary xanthuria and exfoliative dermatitis, the uric acid decreases. At present, the detection of uric acid is mostly carried out by enzymatic method, using the function of uricase, ultraviolet spectrometry, oxygen electrode method, coupled peroxidase (POD) colorimetric method, coupled peroxidase (CAT) and ald...

AI Technical Summary

Problems solved by technology

However, because the activity of the enzyme will be interfered by pH, temperature, and chemical reagents, the uric acid detection reagent has the problem of poor stability, which affects clinical use.

[0003] At present, there is relatively little information on the stability of uric acid reagents. Patent CN200510111883 provides an enzyme recheck stabilizer, which has a good stabilizing effect on a single enzyme solution, but does not mention whether it can also play a similar role for a specific reagent. Function; Patent CN201410398198.3 mentions a kind of rechecking stabilizer for uric acid assay kit, including gelatin, trehalose, propylene glycol, polyoxyethylene lauryl ether, Proclin300, etc. After adding the stabilizer, the stability of opening the bottle is only Increased to 40 days, and adding the same amount of stabilizer to the dual reagents is not easy to grasp the effect on the stability of the reagents, the operation of the dual reagents is more complicated, and there is no proof of the specific validity period of the reagents after adding the stabilizer

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