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Method on water culture rooting for tissue culture seedlings of Bougainvillea

A hydroponic rooting and bougainvillea technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of long callus subculture time, low outdoor rooting rate, and differentiation into deformed buds, etc. Reduce the induction of callus, reduce production costs, and save time

Active Publication Date: 2017-01-25
CHINA THREE GORGES CORPORATION
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AI Technical Summary

Problems solved by technology

[0002] At present, domestic Bougainvillea tissue culture rapid propagation mainly establishes a rapid propagation regeneration system by inducing callus to differentiate and sprout from stem segments. The proliferation coefficient and rooting rate are low, and the callus subculture takes a long time, slow differentiation, and even differentiate into deformities. Buds or undifferentiated shoots, etc., and the outdoor rooting rate is low

Method used

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Experimental program
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Embodiment Construction

[0018] Below in conjunction with specific embodiment, further illustrate the present invention.

[0019] 1.1 Explants

[0020] Take the bougainvillea bougainvillea that is free from diseases and insect pests, and has full buds and semi-lignified stems with buds (including 2-3 full buds).

[0021] 1.2 Disinfection of explants

[0022] First, the base of the collected bougainvillea with buds and stems was treated with a concentration of 1mg / LGA 3 Soak it for 1 hour → soak it in 0.5% washing powder solution for 10 minutes → wash it in running water for 1 hour → soak it in 75% alcohol for 30 seconds on the ultra-clean workbench, then disinfect it with 0.1% mercury liter for 18 minutes → Rinse with sterile water for 5-6 times → place the explants on sterile filter paper to absorb excess water to obtain sterilized explants.

[0023] 1.3 Indoor culture conditions during cluster bud induction: temperature 24±1°C, humidity 80±5%, light 14-16h / d, light intensity 2000-2500Lx.

[0024...

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PUM

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Abstract

The invention discloses a method on water culture rooting for tissue culture seedlings of Bougainvillea. The soft stem of Bougainvillea is collected for explant, which is inoculated in the culture medium Ms + 6-ba2.0 mg . L-1 + Naa0.5 mg . L-1 + sucrose 25g . L-1 + agar 5.8g . L-1,to induce adventitious bud. And after seeding is strengthened, it's conducted the water-cultured rooting and then directly conducted the domestication and transplantation. Cycle, which could simplify the experimental procedures,reduce production costs, improve efficiency and could be suitable for large-scale plant nursery.

Description

technical field [0001] The invention relates to a plant tissue culture propagation technology, in particular to a method for hydroponic rooting of bougainvillea tissue culture seedlings. Background technique [0002] At present, domestic Bougainvillea tissue culture rapid propagation mainly establishes a rapid propagation regeneration system by inducing callus to differentiate and sprout from stem segments. The proliferation coefficient and rooting rate are low, and the callus subculture takes a long time, slow differentiation, and even differentiate into deformities. Buds or undifferentiated buds, etc., and the outdoor rooting rate is low. Contents of the invention [0003] The object of the present invention is to provide a method for hydroponic rooting of bougainvillea tissue culture seedlings, which can shorten the tissue culture cycle, simplify the experimental procedures, reduce production costs, improve work efficiency, and is suitable for large-scale seedling culti...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G31/00A01H4/001A01H4/008
Inventor 李翩翩张国禹黄桂云邱利文吴笛吴锦华张海波马晓波胡梅香杨兰芳汪磊
Owner CHINA THREE GORGES CORPORATION
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