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Screening method for active components of natural drug

A technology of natural medicines and active ingredients, applied in the field of medicine, can solve the problems of environmental pollution of extractants, waste of extractants and materials, etc.

Inactive Publication Date: 2017-02-15
YICHUN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a screening method for the active ingredients of natural medicines, aiming at solving the waste of extractants and materials in the existing methods for extracting active ingredients of natural medicines. defect problem

Method used

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  • Screening method for active components of natural drug
  • Screening method for active components of natural drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0177] Example 1: Screening of Hexokinase Inhibitory Active Components in the Extract of Squilla Sinensis

[0178] 1. Determination of ATP and ADP by LC-MS

[0179] Chromatographic column: Agilent Extend C-18 (3.1×100mm, 3μm; Agilent, Palo Alto, CA, USA); mobile phase: 5mM ammonium acetate (pH7.5):methanol=6:4; flow rate: 0.3mL / min; Column temperature: 20°C; Injection volume: 10 μL Ion source: Liquid chromatography-mass spectrometry interface: Electrospray interface (ESI), positive ion mode (positiveion); Drying gas flow rate: 10mL / min; Drying gas temperature: 300 ℃; atomizing gas pressure: 30psi (upper limit 60psi); capillary voltage: 3000V; mass analyzer: detection method: selected ion monitoring (SIM); detection interval: 0-1.6min; fragment voltage: 100V; monitoring ion: ATP, Pseudo-molecular ion peak (m / z 508); ADP, quasi-molecular ion peak (m / z 428)

[0180] Specificity: The ATP retention time is 1.39 minutes, and the ADP retention time is 1.40 minutes. Compared with th...

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Abstract

The invention discloses a screening method for the active components of a natural drug. According to the method, the characteristics of the microscopic image of the natural drug are extracted; a hexokinase catalytic reaction with ATP as a substrate is carried out; and through quantitative analysis of ATP and a product ADP, active components with hexokinase inhibition effect in a traditional Chinese medicinal material or natural drug are screened out. The method provided by the invention is small in the usage amount of extract and biochemical reagents, short in screening period, rapid, simple, convenient, economical and effective; and the method is applicable to screening of active components with hexokinase activity inhibition effect in a traditional Chinese medicinal material or natural drug.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a screening method for active ingredients of natural medicines. Background technique [0002] Traditional Chinese medicine or natural medicine compound is the main form of clinical medicine of traditional Chinese medicine, has a long-term folk application basis, and is the main source of new drug research and development. Modern medical research has shown that due to the complex and diverse components of traditional Chinese medicine or natural medicine compound, they exert therapeutic effects in the form of multiple channels, multiple targets, and multiple receptors. At present, the method of screening active ingredients from traditional Chinese medicine or natural medicine compound is mainly the extraction and separation method, which is combined with pharmacology to make a positive contribution to the discovery and separation of active ingredients; in recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/08G06T5/00G06T7/00G06T11/00
CPCG06T11/003G01N30/06G01N30/08G01N30/88G01N2030/8809G01N2030/027G01N2030/062G06T2207/20084G06T5/70
Inventor 周伟华
Owner YICHUN UNIVERSITY
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